将紫罗兰酮与甲醇按1∶4~1∶5(质量比)比例,放入三口反应瓶中搅匀,开冷冻机打循环使反应温度在-30±5℃。使氧气通入臭氧发生器,将产生的臭气返入反应瓶中,返入臭氧量为 n(紫罗兰酮)∶n(臭氧)=1∶1.15~1∶1.50,反应4~6h。反应完成,在反应瓶 中,加入还原剂、酸及水搅匀,在-30℃~20℃反应0.5h,调温到20~25℃反应1h。反应结束后加入有机溶剂进行萃取、分离、回收有机溶剂得BETA-环柠檬醛粗产品,经真空精馏,得到一定纯度的BETA-环柠檬醛。
β-Cyclocitral is derived from the catalytic breakdown of β-carotene, and evidence indicates that it is produced by the activation of a specific carotene oxygenase by all species of the bloom-forming cyanobacterium Microcystis. β-cyclocitral affects grazer behavior.