EGS Crosslinker
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- CAS-Nr.
- 70539-42-3
- Englisch Name:
- EGS Crosslinker
- Synonyma:
- EGS;EGNHS;HI-EFF-2B;LAC-4R-886;EGS Crosslinker;ethyleneglycolylbis(succinimidylsuccinate;Ethlene glycol bis(succinimidyl succinate);ETHYLENE GLYCOL BIS(SUCCINIMIDYL SUCCINATE);DI(N-SUCCINIMIDYL) ETHYLENE GLYCOL DISUCCINATE;Ethylene glycol bis(succiniMidylsuccinate)(EGS)
- CBNumber:
- CB5722624
- Summenformel:
- C18H20N2O12
- Molgewicht:
- 456.36
- MOL-Datei:
- 70539-42-3.mol
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EGS Crosslinker Eigenschaften
- Siedepunkt:
- 603.4±65.0 °C(Predicted)
- Dichte
- 1.52±0.1 g/cm3(Predicted)
- storage temp.
- 2-8°C
- Löslichkeit
- acetic acid: water (1:1): ≤50mg/mL
- Aggregatzustand
- powder
- BRN
- 9167119
- InChI
- InChI=1S/C18H20N2O12/c21-11-1-2-12(22)19(11)31-17(27)7-5-15(25)29-9-10-30-16(26)6-8-18(28)32-20-13(23)3-4-14(20)24/h1-10H2
- InChIKey
- QLHLYJHNOCILIT-UHFFFAOYSA-N
- SMILES
- O(N1C(CCC1=O)=O)C(=O)CCC(=O)OCCOC(=O)CCC(=O)ON1C(CCC1=O)=O
EGS Crosslinker Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is commonly used as a crosslinker in chemical reactions. It is a homobifunctional crosslinking reagent that is reactive with bromoacetate amines and sulfhydryls.
Biochem/physiol Actions
Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is a homobifunctional, cleavable cross-linking reagent. It is mainly coupled to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). The linkage provides a 12-atom spacer.
Properties and Applications
EGS crosslinker, Ethylene glycolbis(succinimidyl succinate), is a water-insoluble homobifunctional crosslinker that must be dissolved in DMSO or DMF before addition to an aqueous reaction. EGS crosslinking takes place through amine-reactive NHS esters at both ends of a 16.1-angstrom spacer arm. The 12-atom spacer arm of the EGS crosslinking reagent is cleavable and the spacer arm is cleaved by hydroxylamine at pH 8.5 for 3-6 hours at 37°C. The EGS protein crosslinker possesses amino reactive NHS esters on both ends of the molecule and can be used to label intracellular proteins since the molecule is cell membrane permeable. EGS crosslinker is often used in ChIP assays to determine DNA-protein binding interactions. The directly water-soluble analog of EGS is Sulfo-EGS.
EGS Crosslinker Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
EGS Crosslinker Anbieter Lieferant Produzent Hersteller Vertrieb Händler.
Global( 103)Lieferanten
70539-42-3()Verwandte Suche:
- ethyleneglycolylbis(succinimidylsuccinate
- ETHYLENE GLYCOL BIS-(SUCCINIC ACID*N-HYD ROXYSUCCINI
- Ethlene glycol bis(succinimidyl succinate)
- Ethyleneglycol-O,O''-bis-(succinic acid N-hydroxysuccinimide ester)
- Di(N-succinimidyl) ethylene glycol disuccinate, EGS, Ethylene glycol bis(succinimidyl succinate), Ethylene glycol disuccinate di(N-succinimidyl) ester
- Ethylene Glycol Bis(succinic Acid N-Hydrosuccinimide Ester)
- HI-EFF-2B
- LAC-4R-886
- Butanoic acid, 4-[(2,5-dioxo-1-pyrrolidinyl)oxy]-4-oxo-, 1,2-ethanediyl ester
- Ethylene glycol bis(succininic acid) N-hydroxysucciniMide ester
- Ethylene glycol bis(succiniMidylsuccinate)(EGS)
- EGS Crosslinker (ethylene glycol bis(succinimidyl succinate))
- DI(N-SUCCINIMIDYL) ETHYLENE GLYCOL DISUCCINATE
- EGS
- ETHYLENE GLYCOL-BIS(SUCCINIC ACID N-HYDROXYSUCCINIMIDE ESTER)
- ETHYLENE GLYCOL BIS(SUCCINIMIDYL SUCCINATE)
- ETHYLENE GLYCOL DISUCCINATE DI(N-SUCCINIMIDYL) ESTER
- ethane-1,2-diyl bis{4-[(2,5-dioxopyrrolidin-1-yl)oxy]-4-oxob...
- Butanedioic acid, 1,1'-(1,2-ethanediyl) 4,4'-bis(2,5-dioxo-1-pyrrolidinyl) ester
- 4-O-(2,5-dioxopyrrolidin-1-yl) 1-O-[2-[4-(2,5-dioxopyrrolidin-1-yl)oxy-4-oxobutanoyl]oxyethyl] butanedioate
- ethylene glycol-bis(succinic acid n-hydroxysuccinimide
- EGNHS
- Bis(2,5-dioxopyrrolidin-1-yl) O,O'-ethane-1,2-diyl disuccinate
- EGS Crosslinker
- ethane-1,2-diyl bis{4-[(2,5-dioxopyrrolidin-1-yl)oxy]-4-oxobutanoate}
- Bis(2,5-dioxopyrrolidin-1-yl) O,O'-ethane-1,2-diyl disuccinate
- 70539-42-3
- C18H20N2O12
- Active Esters/Additives
- Coupling
- Synthetic Reagents
- homoXlink
- Homobifunctional Crosslinker
