ChemicalBook
Chinese Japanese Germany

SEPHADEX G-50

Description Uses
SEPHADEX G-50
SEPHADEX G-50
CAS No.
9048-71-9
Chemical Name:
SEPHADEX G-50
Synonyms
PDXTM G.F.;SEPHADEX G-50;SEPHADEX(R) G-50;PDX GF 50 (50-150);SEPHADEX G-50 FINE;PDX GF 50 (100-300);SEPHADEX G-50 MEDIUM;Sephadex G-50 60-100;SEPHADEX G-50 COARSE;Sephadex G-50 200-400
CBNumber:
CB3728066
Molecular Formula:
NULL
Formula Weight:
0
MOL File:
Mol file

SEPHADEX G-50 Properties

SAFETY

WGK Germany  3

SEPHADEX G-50 Chemical Properties,Uses,Production

Description

Sephadex G-50 Superfine is a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine´s small bead size give higher efficiency.
  1. Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  2. Suitable for purification of DNA from small molecules by gel filtration
  3. Small bead size for shorter diffusion distances
  4. Smallest bead size in the Sephadex G-50 range
Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Uses

The media used for gel exclusion chromatography include dextran (Sephadex™), polyacrylamide (Bio-Gel P™) and dextran-polyacrylamide (Sephacryl™) and agarose (Sepharose™ and BioGel A™). Each is available with a variety of different ranges of pore size in the beads, permitting separation of macromolecules of different size.
A gel with a smaller range of pore sizes (and hence a smaller range over which it can separate macromolecules on the basis of their size) will give a higher resolution; a gel with a wider range will give lower resolution, but will permit fractionation of a larger range of sizes as an initial step when the approximate molecular mass of the enzyme is not known.
The particle size of the gel beads (the mesh size) also affects resolution; smaller beads permit higher resolution, but a lower flow rate through the column (and hence a slower separation).
The table shows the useful range for the most commonly used gel filtration media - the lower and upper molecular sizes (in kDa) over which they can be used to separate macromolecules. The upper limit is known as the exclusion limit of the gel - the size above which proteins will elute in the void volume of the column.
  lower limit upper limit
dextran gels
Sephadex G-50 1.5 30
Sephadex G-75 3 80
Sephadex G-100 4 150
Sephadex G-150 5 300
Sephadex G-200 5 600
polyacrylamide gels
Bio-Gel P-10 1.5 20
Bio-Gel P-30 2.5 40
Bio-Gel P-60 3 60
Bio-Gel P-100 5 100
Bio-Gel P-150 15 150
BioGel P-200 30 200
Bio-Gel P-300 60 400
dextran-polyacrylamide gels
Sephacryl S-200 5 250
Sephacryl S-300 10 1500
Sephacryl S-400 20 8000
agarose gels
Sepharose 6B 10 4000
Sepharose 4B 60 20,000
Sepharose 2B 70 40,000
Bio-Gel A-0.5 10 500
Bio-Gel A-1.5 10 1500
Bio-Gel A-5 10 5000
Bio-Gel A-15 40 15,000
Bio-Gel A-50 100 50,000

SEPHADEX G-50 Preparation Products And Raw materials

Raw materials

Preparation Products


SEPHADEX G-50 Suppliers

Global( 37)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
future industrial shanghai co., ltd 021-61552785,400-0066-400
021-55660885 sales@jonln.com China 2090 65
Nanjing Chemlin Chemical Co., Ltd 025-83697070
+86-25-83453306 sales@chemlin.com.cn China 20013 64
BEST-REAGENT 028-85973071 028-85973075
028-85973075 service@best-reagent.com China 9966 57
Tianjin heowns Biochemical Technology Co., Ltd. 400 638 7771
sales@heowns.com China 14544 57
Sinopharm Chemical Reagent Co,Ltd. 86-21-63210123
86-21-63290778 86-21-63218885 sj_scrc@sinopharm.com China 9977 79
ShangHai LanJi Science and Technology Development Co., Ltd. 021-56373608/66107288/13391393747
021-66107288 liujun@lanji.cn/QQ493513614 China 299 62
Dalian Meilun Biotech Co., Ltd. 0411-66771943;0411-66771942
0411-66771945 sales@meilune.com China 4801 58
ShangHai YuanYe Biotechnology Co., Ltd. 021-61312847 qq:2797782341
021-55068248 2797782341@qq.com China 7590 60
Beijing HuaMeiHuLiBiological Chemical 010-56205725;010-86181995
010-65763397 waley188@sohu.com China 12397 58
Shanghai T&W Pharmaceutical Co., Ltd. +86 21 61551611
+86 21 50676805 contact@trustwe.com China 9971 58

9048-71-9(SEPHADEX G-50)Related Search:


  • DNA&RNA Purification
  • Molecular Biology
  • Reagents
  • PDXTM G.F.
  • Gel Filtration Media
  • Sephadex(R) G-50 Fine
  • Cross-inked dextran gel G-50
  • Sephadex G-50 100-200
  • Sephadex G-50 200-400
  • Sephadex G-50 60-100
  • SEPHADEX G-50 MEDIUM
  • SEPHADEX G-50 SUPERFINE
  • SEPHADEX(R) G-50
  • DNA & RNA Purification
  • Gel Filtration Media
  • Gel Filtration Chromatography
  • Sephadex
  • Sephadex® G-50
  • Resins
  • Resins and Media
  • Reagents
  • Protein Analysis
  • Protein Chromatography
  • Proteomics and Protein Expression
  • Molecular Biology
  • sephadex(R) G-50 fine for chromato-graphy
  • sephadex(R) G-50 medium for chromato-graphy
  • sephadex(R) G-50 superfine for chromato-graphy
  • Cross-linked dextran gel G-50
  • SEPHADEX(R) G-50 MEDIUM FOR CHROMATO- GRAPHY, 50-150 UM*
  • SEPHADEX G-50, FOR GEL FILTRATION, 100-300MICRON
  • SEPHADEX G-50 MEDIUM DNA GRADE
  • SEPHADEX G-50, FOR GEL FILTRATION, 50-15 0MICRON
  • SEPHADEX(R) G-50 FINE FOR CHROMATO- GRAPHY, 20-80 UM*
  • SEPHADEX G-50, FOR GEL FILTRATION, 20-50MICRON
  • D(+)-GLUCOSE MOLECULAR BIOLOGY REAGENT
  • SEPHADEX(R) G-50 SUPERFINE FOR CHROMATO- GRAPHY, 20-50 UM*
  • SEPHADEX G-50, FOR GEL FILTRATION, 20-80MICRON
  • SEPHADEX G-50 FINE DNA GRADE
  • SEPHADEX G-50, SUPERFINE 20-50MICRON
  • SEPHADEX G-50, FINE 20-80MICRON
  • SEPHADEX G-50, MEDIUM 50-150MICRON
  • SEPHADEX G-50, COARSE 100-300MICRON
  • Sephadex G-50
  • Gel Filtration Chromatography
  • Gel Filtration MediaGel Filtration Media
  • Protein Chromatography
  • Sephadex
  • 9048-71-9
  • BioChemical
  • Analytical Chromatography Product Catalog
  • PDX GF 50 (100-300)
  • PDX GF 50 (50-150)
  • SEPHADEX G-50
  • SEPHADEX G-50 COARSE
  • SEPHADEX G-50 FINE
Copyright 2017 © ChemicalBook. All rights reserved