ピリドキサール塩酸塩 化学特性,用途語,生産方法
外観
白色〜うすい黄褐色, 結晶性粉末〜粉末
溶解性
水溶状：試験適合 (0.5g/10ml)水に溶ける。
用途
ビタミン B6 の酸化物です。
ビタミン B6 作用を示します
用途
ビタミン B6 の酸化物です。
ビタミン B6 作用を示します。
用途
ピリドキサール定量用標準品。
説明
Pyridoxal hydrochloride is a hydrochloride salt formed by combining pyridoxal with hydrochloric acid. It is also a derivative of pyridinium salt and vitamin B6. It has important roles in E. coli metabolites, Saccharomyces cerevisiae metabolites, cofactors, human metabolites and mouse metabolites. It can be used as a calibrator for liquid chromatography of food samples, a component of culture media for lactic acid bacteria (LAB) and foodborne pathogens (FBP) (component of arginine decarboxylase and lysine decarboxylase).
化学的特性
White to off-white crystalline powder
使用
Pyridoxal hydrochloride is used in labeling of amino acids for their detection. It finds application in neurotransmitters, sphingolipids and aminolevulinic acid. It is also used as a nutritional agent. It is also applied in research studies like biochemical, drugs and in media supplements.
定義
ChEBI: Pyridoxal hydrochloride is a hydrochloride obtained by combining pyridoxal with one molar equivalent of hydrochloric acid. It has a role as an Escherichia coli metabolite, a Saccharomyces cerevisiae metabolite, a cofactor, a human metabolite and a mouse metabolite. It is a hydrochloride, a pyridinium salt and a vitamin B6. It contains a pyridoxal(1+).
一般的な説明
Pyridoxal is a heterocyclic compound, weighing 167.2 Da. It is one of the natural forms of vitamin B
6. Pyridoxal is found to be less stable than pyridoxine, hence heating might result in the loss of its action. Pyridoxal has a wide variety of sources and is present in both plants and animals. Pyridoxal serves as an efficient precursor for coenzymes : pyridoxal phosphate and pyridoxamine phosphate.
安全性プロファイル
Poison by
intramuscular, intravenous, and
intraperitoneal routes. Moderately toxic by
ingestion and subcutaneous routes. When
heated to decomposition it emits very toxic
fumes of NOx and HCl. See also
ALDEHYDES.
合成
In
the 25mL reaction flask with agitator and thermometer, add successively
0.085g (0.5mmol) 2,2,6,6-tetramethyl piperidine-nitrogen-oxyradical,
0.29g (2mmol) cupric bromide, 0.40g (5mmol) pyridine and 2.06g (10mmol)
pyridoxinehydrochloride. Add
water 5mL, temperature is controlled at 30 DEG C, opens and stirs.Then
drip 2.0g (18mmol) 30% hydrogen peroxide, control temperature of
reaction at 30～35 DEG C simultaneously.After dropwising, insulation
continues to stir 1～2 hour at 30～35 DEG C, and gained reaction solution
reaches 99% (liquid-phase condition: chromatographic column is XDB-G8
250 × 4.6 mm 5um through Liquid Detection pyridoxol transformation
efficiency.Moving phase is methyl alcohol: buffer=15: 85; Buffer:0.04%
sodium pentanesulfonate is adjusted PH to 3 with Glacial acetic acid;
Detection wavelength is 284nm), reaction preference is 98%.
In
addition, get about 5mL reaction solution and directly go up silicagel
column, eluent is methylene dichloride: methyl alcohol=20: 1, obtain
final elutriant through gradient elution, and be concentrated into
dryly, can obtain white solidpyridoxalhydrochloride, fusing point 164.1-164.8 DEG C, nuclear-magnetism characterizes as follows: 1hNMR (400MHz, DMSO), δ: 8.27 (s, 1H, CHO), 6.63 (d, 1H, CH), 5.00-5.16 (m, 2H, CH2), 2.62 (s, 3H, CH3).
Because pyridoxalhydrochloride is unstable, difficult separation, reaction solution
then adds p-ethoxyaniline 1.37g (10mmol), treated yellow Schiff alkali
(IX) 2.10g that obtains without separating.The total recovery of
two-step reaction is 88%.
純化方法
Dissolve it in water and adjust the pH to 6 with NaOH. Set aside overnight to crystallise. The crystals are washed with cold water, dried in a vacuum desiccator over P2O5 and stored in a brown bottle at room temperature. The free base is then converted to the hydrochloride with one equivalent of HCl. [Fleck & Alberty J Phys Chem 66 1678 1962, Beilstein 21/13 V 44.]
ピリドキサール塩酸塩 上流と下流の製品情報
原材料
準備製品