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Sigma-Aldrich
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Product Name:alpha-Ketoglutarate Dehydrogenase from porcine heart
CAS:9031-02-1
Purity:buffered aqueous glycerol solution, 0.1-1.0 units/mg protein Package:20UN Remarks:K1502-20UN
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Guangzhou Tomums Life Science Co., Ltd.
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020-31155029 18902330969 |
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sales@tomums.cn |
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Product Name:α-Ketoglutarate Dehydrogenase from porcine heart
CAS:9031-02-1
Package:20UN;50UN
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| Company Name: |
Shanghai Guchen Biotechnology Co., LTD
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021-34675735 19147740836 |
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1986399151@qq.com |
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Product Name:α-Ketoglutarate Dehydrogenase, from porcine heart
CAS:9031-02-1
Purity:0.1-1.0 units/mg protein Package:10U
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Creative Enzymes
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1-516-855-7709 |
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Product Name:oxoglutarate dehydrogenase (succinyl-transferring)
CAS:9031-02-1
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| Company Name: |
Riedel-de Haen AG
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800 558-9160 |
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CAS:9031-02-1
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| | ALPHA-KETOGLUTARATE DEHYDROGENASE Basic information |
| Product Name: | ALPHA-KETOGLUTARATE DEHYDROGENASE | | Synonyms: | ALPHA-KETOGLUTARATE DEHYDROGENASE;A-ketoglutarate dehydrogenase from*porcine heart;multienzyme 2-oxoglutarate dehydrogenase complex;α-ketoglutarate dehydrogenase from porcine heart;MULTIENZYME 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, (1) INCLUDING EC 1.2.4.2;α-Ketoglutarate Dehydrogenase;alpha-Ketoglutarate Dehydrogenase from porcine heart;α-Ketoglutarate Dehydrogenase, from porcine heart | | CAS: | 9031-02-1 | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | | | Mol File: | Mol File | ![ALPHA-KETOGLUTARATE DEHYDROGENASE Structure]() |
| | ALPHA-KETOGLUTARATE DEHYDROGENASE Chemical Properties |
| storage temp. | -20°C | | form | buffered aqueous glycerol solution |
| | ALPHA-KETOGLUTARATE DEHYDROGENASE Usage And Synthesis |
| Uses | α-Ketoglutarate Dehydrogenase from the porcine heart has been used:
- to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cells
- to measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondria
- as a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons
| | General Description | α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2). | | Biochem/physiol Actions | α-Ketoglutarate dehydrogenase (α-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of α-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. α-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, α-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of α-KGDH cause several neurodegenerative diseases such as Alzheimer′s disease. |
| | ALPHA-KETOGLUTARATE DEHYDROGENASE Preparation Products And Raw materials |
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