生物素标记 肿瘤坏死因子抗体

Goat Anti-Human Tumor Necrosis Factor-α (TNF-α) recognizes Human TNF-α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.Purified Recombinant Human TNF-α (Accession # P01375)Reacts with: HumanBiotinIHC (NBF/Par.)/Western Blotting/ELISA Sandwich Assay

Western blot - Anti-TNF alpha antibody (Biotin) (ab271247)

Western blot analysis of varying levels of Recombinant human TNF-α protein using ab271247 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

TNF-α is a 17.5 kD protein that mediates inflammation and immunity caused by the invasion of viruses, bacteria, and parasites by initiating a cascade of cytokines that increase vascular permeability, thus bringing macrophages and neutrophils to the site of infection. TNF-α secreted by the macrophage causes the blood to clot which provides containment of the infection. TNF-α binding to surface receptors brings about various biologic activities that include cytolysis and cytostasis of many tumor cell lines In vitro, hemorraghic necrosis of tumors In vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.双抗体夹心法测定标本中肿瘤坏死因子水平。用纯化的肿瘤坏死因子抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入肿瘤坏死因子，再与HRP标记的肿瘤坏死因子抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的肿瘤坏死因子呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中肿瘤坏死因子浓度。
生物素标记肿瘤坏死因子抗体是一类可以特异性结合肿瘤坏死因子的多克隆抗体，主要用于检测肿瘤坏死因子的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。α肿瘤坏死因子（TNFα）是一种主要由巨噬细胞和单核细胞产生的促炎细胞因子，并参与正常炎症反应和免疫反应。α肿瘤坏死因子在许多病理状态下产生增多，包括败血症、恶性肿瘤、心脏衰竭和慢性炎性疾病。在重症类风湿关节炎患者的血液及关节中都可发现肿瘤坏死因子增多。