荧光素二乙酸盐
中文名称 | 荧光素二乙酸盐 |
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中文同义词 | 二乙酰荧光素;荧光素二乙酸酯(>98.0%(HPLC));二乙酸荧光素(FDA);二乙酸萤光素;荧光素双醋酸酯;醋酸荧光素;FDA,荧光素二乙酸酯;FLUORESCEIN DIACETATE 二乙酸荧光素 |
英文名称 | Fluorescein diacetate |
英文同义词 | 3-Oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate;DI-O-ACETYLFLUORESCEIN;DIACETYLFLUORESCEIN;FDA;FDA [FLUORESCEIN DIACETATE];FLUORESCEIN DI-O-ACETATE;FLUORESCEIN DIACETATE;3’,6’-diacetylfluorescein |
CAS号 | 596-09-8 |
分子式 | C24H16O7 |
分子量 | 416.38 |
EINECS号 | 209-877-6 |
相关类别 | 生化试剂;化学发光;荧光探针,标记,颗粒和染色;色素类;化学试剂;生化试剂-色素类;Colours, Dyes, Indicators & Pigments;marker;Fluorescent;Xanthene;通用生化试剂-生物染色剂与化学指示剂 |
Mol文件 | 596-09-8.mol |
结构式 |
荧光素二乙酸盐 性质
熔点 | 200-203 °C(lit.) |
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沸点 | 494.73°C (rough estimate) |
密度 | 1.2738 (rough estimate) |
折射率 | 1.6810 (estimate) |
储存条件 | −20°C |
溶解度 | 丙酮:可溶25mg/mL |
形态 | 结晶粉末 |
酸度系数(pKa) | 6.4(at 25℃) |
颜色 | 浅黄色 |
酸碱指示剂变色ph值范围 | Weak green uorescence (6.0) to strong green uorescence (7.2) |
水溶解性 | Soluble in chloroform, DMSO, ethanol, and water. |
最大波长(λmax) | 490nm |
BRN | 62575 |
主要应用 | LED devices, petroleum markers, identifying cancerous cells, detecting bacteria, virus, mitochondrial function in neurons, target molecules, living cells, viable Giardia cysts in water samples, apoptosis, evaluating live/dead state of algae, measurement of totalmicrobial activity in soil, treating ischemia |
生物领域应用 | Assessing membrane integrity/permeability;43–50 detecting/counting microorganisms; measuring microbial activity; as a substrate for measuring acylases activity, amidases activity,aminopeptidases activity, arylsulfatases activity,chymotrypsin activity, esterases activity;galactosidases activity, glucosidases activity, lipases activity, phosphatases activity, proteases activity; cell viability assay; cytotoxicity assay; implantable medical devices; as temperature sensor; treating ischemic disease |
CAS 数据库 | 596-09-8(CAS DataBase Reference) |
EPA化学物质信息 | Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)- (596-09-8) |
Fluorescein diacetate (FDA) is an acetylated derivative of the green fluorescent dye fluorescein. Fluorescein diacetate (FDA), a fluorescent probe used for vital staining, is a fluorescently activated by esterolytic activity of human Pi-class glutathione S-transferase (hGSTP1) selectively among various cytosolic GSTs. Fluorescence activation of Fluorescein diacetate susceptible to GST inhibitors is observed in MCF7 cells exogenously overexpressing hGSTP1, but not in cells overexpressing hGSTA1 or hGSTM1. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1. To investigate whether the fluorescence activation is due to hGSTP1 activity, Fluorescein diacetate is incubated with recombinant hGSTP1-1 and GSH in vitro. Remarkable fluorescence activation is observed in the presence of both hGSTP1-1 and GSH, whereas only slight activation is observed in the absence of either of them or when the enzyme is heat inactivated. This suggests that the fluorescence activation of Fluorescein diacetate depends on hGSTP1-1 activity. From the linear relationship between the rate of increase in fluorescence and the hGSTP1-1 concentration, the specific activity of the enzyme for 1 μM Fluorescein diacetate is determined to be 79±15 nmol/min/mg protein. Fluorescein diacetate is applicable as a fluorogenic substrate for evaluating inhibitors of GSTP1-1 in vitro. For Fluorescein diacetate as a substrate, both Ethacrynic acid (EA) and NBDHEX suppress the hGSTP1-1-dependent fluorescent increase in a concentration-dependent manner, with IC 50 s of 3.3±0.5 μM and 0.61±0.04 μM, respectively.
安全信息
危险品标志 | Xi |
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危险类别码 | 36/37/38 |
安全说明 | 24/25-36/37/39-27-26 |
WGK Germany | 3 |
TSCA | Yes |
海关编码 | 32129000 |
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