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| | STO-609 (acetate) Basic information |
| Product Name: | STO-609 (acetate) | | Synonyms: | PubChem ID: 16760660;STO-609;STO 609;STO609;CS-2405;7-Oxo-7H-benzo[de]benzo[4,5]imidazo[2,1-a]isoquinoline-3-carboxylic Acid Acetate (1:1);7-oxo-7H-benzo[de]benzo[4,5]imidazo[2,1-a]isoquinoline-3-carboxylic acid;7H-Benzimidazo[2,1-a]benz[de]isoquinoline-3-carboxylic acid, 7-oxo-, acetate (1:1) | | CAS: | 1173022-21-3 | | MF: | C21H14N2O5 | | MW: | 374.35 | | EINECS: | 604-604-1 | | Product Categories: | | | Mol File: | 1173022-21-3.mol |  |
| | STO-609 (acetate) Chemical Properties |
| Melting point | >300 °C | | storage temp. | 2-8°C | | solubility | Soluble in DMSO (up to 10 mg/ml). | | form | Yellow solid. | | color | Yellow | | Stability: | Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 3 months. |
| | STO-609 (acetate) Usage And Synthesis |
| Description | STO-609 (1173022-21-3) is a selective inhibitor of Ca2+-calmodulin-dependent protein kinase kinase (Ki = 80 and 15 ng/ml for inhibition of CaM-KKα and CaM-KKβ respectively).1 Binds to the? ATP-binding site.2 Displays > 80-fold selectivity over CaMK1, CaMK2, CaMK4, MLCK, PKC, PKA and p42 MAPK. Important tool for probing distinct CaMK pathways in LTP.3 Reduces starvation-induced autophagosomal membrane formation.4 Reverses age-associated decline in bone mass.5 Stimulates osteoblast formation, inhibits osteoclast differentiation.6 | | Uses | STO-609 is a cell-permeable inhibitor of calcium/calmodulin-dependent kinase kinases (CaMKK) isoforms CaMKKα and CaMKKβ. It is used to evaluate the action of CaMKK isoforms both in vitro and in cells. | | General Description | STO-609 reduces tumorigenicity of liver cancer cells in vivo. TO-609 reduces the activation of AMP (adenosine monophosphate)-activated protein kinase (AMPK) by ionomycin in a dose dependant manner. | | Biochem/physiol Actions | Selective Ca2+/Calmodulin-dependent protein kinase kinase (CaM-KK) antagonist. Inhibits CamKKa and CaMKKb with Ki = 80 and 15 ng/mL, respectively. Does not inhibit downstream CaM kinases (CaMKI and CaMKIV). | | storage | Room temperature (desiccate) | | References | 1) Tokumitsu?et al. (2002), STO-609, a Specific Inhibitor of the Ca2+/Calmodulin-dependent Protein Kinase Kinase;?J. Biol. Chem. 277 15813
2) Tokumitsu?et al. (2003) A single amino acid difference between alpha and beta Ca2+/calmodulin-dependent protein kinase kinase dictates sensitivity to the specific inhibitor, STO-609;?J. Biol. Chem. 278 10908
3) Redondo et al. (2010) Synaptic tagging and capture: differential role of distinct calcium/calmodulin kinases in protein synthesis-dependent long-term potentiation;?J. Neurosci. 30 4981
4) Pfisterer et al. (2011) Ca+2/calmodulin –dependent kinase (CaMK) signaling via CaMKI and AMP-activated protein kinase contributes to the regulation of WIPI-1 ar the onset of autophagy; Mol. Pharmacol. 80 1066
5) Pritchard et al. (2015) Inhibition of CaMKK2 reverses age-associated decline in bone mass; Bone, 75 120
6)Cary?et al. (2013) Inhibition of Ca+2/Calmodulin-dependent protein kinase kinase 2 stimulates osteoblast formation and inhibits osteoclast differentiation;?J. Bone Miner. Res. 28 1599
7) Matsukawa et al. (2017) Upregulation of skeletal muscle PGC-1α through the elevation of cyclic AMP levels by Cyanidin-3-glucoside enhances exercise performance; Sci. Rep., 7 44799 |
| | STO-609 (acetate) Preparation Products And Raw materials |
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