| Company Name: |
Sigma-Aldrich
|
| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
Purity:Liquid Substrate System Package:100mL Remarks:A3219
|
| Company Name: |
Creative Enzymes
|
| Tel: |
1-516-855-7709 |
| Email: |
info@creative-enzymes.com |
| Products Intro: |
Product Name:2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
|
|
| Hazard Codes | O | | Risk Statements | 8 | | Safety Statements | 17-36 | | WGK Germany | 3 |
| Uses | 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) has been used as a peroxidase substrate in ELISA experiment. This substrate gives a green color soluble end product. It has also been used as a colloidal preparation spread over the standard media plates, to evaluate the lignin oxidation activity. Presence of green coloration of the substrate exhibits microbial laccase activity. | | General Description | The product is supplied as a ready-to-use one component substrate for peroxidase consisting 2,2′-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid) in an acidic buffer. The substrate is colorless before reacting with peroxidase and produces a blue-green color reaction product after reacting with it. 1% sodium dodecyl sulfate (SDS) may be used to stop the reaction during end-point assays. Since this substrate produces a soluble reaction product, it is not recommended for immunoblotting or histochemical applications. | | Biochem/physiol Actions | 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a cation and undergoes oxidation in the presence of hydrogen peroxide. The oxidized ABTS is a blue-green product and is measurable colorimetrically. A combination of ABTS, hydrogen peroxide and myoglobin is a standard system employed for measuring total antioxidant capacity(TAC) from samples. ABTS method is flexible at varying pH and most widely used. ABTS is a substrate for phenol oxidase enzyme. |
| | ABTS Preparation Products And Raw materials |
|