组蛋白H3.3抗体

Histone H3.3 antibody LS-C383945 is an unconjugated rabbit polyclonal antibody to Histone H3.3 (10-90 aa) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.RabbitH3 histone, family 3AHuman,Mouse ,RatUnconjugatedELISA,WB

Western blot of Histone H3.3 antibody of LSM bio (10‑90 aa, IHC, IF, WB) LS‑C383945

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.双抗体夹心法测定标本中组蛋白H3.3水平。用纯化的组蛋白H3.3抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入组蛋白H3.3，再与HRP标记的组蛋白H3.3抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的组蛋白H3.3呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中组蛋白H3.3浓度。
组蛋白H3.3抗体是一类可以特异性结合组蛋白H3.3的多克隆抗体，主要用于检测组蛋白H3.3的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。