2-Hydroxybenzylamine synthesis
- Product Name:2-Hydroxybenzylamine
- CAS Number:932-30-9
- Molecular formula:C7H9NO
- Molecular Weight:123.15
1. Dry the roots, stems, and seeds of buckwheat separately. Grind them into a powder using liquid nitrogen.
2. Mix the powder with deionized water and adjust the pH of the solution to 5.0. Add an enzyme mixture consisting of cellulase and pectinase, with a weight ratio of 2:1 to the powder. Perform enzymatic hydrolysis at a temperature of 25°C for 40 minutes. Filter the mixture after hydrolysis.
3. Mix the filter residue obtained in step 2 with 95% ethanol using a solid-liquid ratio of 1g:10mL. Perform ultrasonic extraction at an ultrasound power density of 100W/cm2, ultrasound frequency of 30kHz, extraction temperature of 20°C, and extraction time of 30 minutes. Repeat the extraction process three times, filter the extracts, and combine them.
4. Concentrate the supernatant obtained in step 3 under reduced pressure to obtain a Chemicalbook extract. Add a 3 wt% sodium hydroxide solution to dissolve the extract. Filter the solution to obtain the filtrate.
5. Adjust the filtrate obtained in step 4 to pH 3 using hydrochloric acid, resulting in an adjusted solution. Pass the adjusted solution through an NDA-150 resin column at a temperature of 20°C and a flow rate of 10 BV/h until saturated adsorption is achieved.
6. After reaching saturated adsorption, elute the filtrate obtained in step 4 with an 8 wt% sodium hydroxide solution at 50°C and a flow rate of 2 BV/h. Collect twice the volume of the column volume. Then, elute the filtrate obtained in step 5 with a 3 wt% sodium hydroxide solution at 50°C and a flow rate of 2 BV/h. Collect once the volume of the column volume. Lastly, elute the filtrate obtained in step 5 with water at 60°C and a flow rate of 3 BV/h. Collect once the volume of the column volume. Combine the three eluents.
7. Concentrate the combined eluents under reduced pressure and recrystallize them using edible alcohol. Separate the crystals to obtain 2-hydroxybenzylamine.
611-20-1
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932-30-9
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Yield:932-30-9 97%
Steps:
44.A A.
A. 2-(Aminomethyl)phenol 2-Hydroxybenzonitrile (1.2 g, 0.010 mol) was used in the process for preparing compound A of Example 38 to provide compound A (1.2 g, 97%), MS: (M+H)+ 124+.
References:
EP696593,1996,A2
6850-57-3
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932-30-9
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94-67-7
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932-30-9
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90-02-8
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932-30-9
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390427-07-3
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932-30-9
163 suppliers
$10.00/250mg