ChemicalBook CAS DataBase List Rebaudioside A

Rebaudioside A synthesis

5synthesis methods

Product Name:Rebaudioside A
CAS Number:58543-16-1
Molecular formula:C44H70O23
Molecular Weight:967.01

57817-89-7 Synthesis

57817-89-7
518 suppliers
$5.00/1g

57-50-1 Synthesis

57-50-1
561 suppliers
$5.00/10g

58543-16-1
252 suppliers
$5.00/25mg

Yield:58543-16-1 78%

Reaction Conditions:

with Arabidopsis thaliana sucrose synthase gene;UDP-glucosyltransferase from Stevia rebaudiana, recombinant;magnesium chloride in aq. phosphate buffer; pH=7.2 at 30; for 30 h;Enzymatic reaction;Time;

Steps:

Enzymatic synthesis of rebaudioside A with UGT76G1 and AtSUS1
After pEUGT-SUS expressionin E. coli, the cells were harvested and washed twice in potassium phosphate buffer (100 mM, pH 7.2). A lysate containing soluble proteins was obtained by sonication on an ice-water bath and subsequent clarification by centrifugation at 4 °C. The supernatant was used as the crude extract. Total protein concentrations were measured by the Bradford assay using bovine serum albumin (BSA) as a standard.23) The substratestevioside was incubated with aliquots of the crude extract at 30 °C in a reaction mixture containing varying concentrations of UDP-glucose or UDP, sucrose, and 3 mM MgCl2 in potassium phosphate buffer (100 mM, pH 7.2) for synthesis of rebaudioside A.

References:

Wang, Yu;Chen, Liangliang;Li, Yan;Li, Yangyang;Yan, Ming;Chen, Kequan;Hao, Ning;Xu, Lin [Bioscience, Biotechnology and Biochemistry,2016,vol. 80,# 1,p. 67 - 73]

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57817-89-7 Synthesis

57817-89-7
518 suppliers
$5.00/1g

58543-16-1
252 suppliers
$5.00/25mg

References