| Identification | Back Directory | [Name]
5-TAMRA Azide | [CAS]
1006592-61-5 | [Synonyms]
5-TAMRA N3
5-TAMRA Azide
5-TAMRA-C6-azide
5-TAMRA Azide,5-TAMRA N3
5-((6-azidohexyl)carbamoyl)-2-(3,6-bis(dimethylamino)xanthylium-9-yl)benzoate
5-((6-azidohexyl)carbamoyl)-2-(6-(dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl)benzoate | [Molecular Formula]
C31H34N6O4 | [MOL File]
1006592-61-5.mol | [Molecular Weight]
554.65 |
| Hazard Information | Back Directory | [Description]
Although the mixed isomers of 5(6)-TAMRA Acid is a preferred, routinely used orange-fluorescent dye for staining proteins, it is rearly used for labeling peptides and nucleotides. Purification of 5(6)-TAMRA labeled peptide and nucleotides might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often required in the conjugation processes. 5-TAMRA is more often used for labeling peptides and proteins. 6-TAMRA is predominately used for labeling nucleotides and sequencing nucleic acids. 5-TAMRA dye is a bright fluorescent label is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line.TAMRA NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules. | [Uses]
5-TAMRA-Azide, is a Fluorescent dye, that can be used for various labeling applications in chemistry.(Abs/Em = 546/579 nm) | [Abs/Em Maxima]
553/575 nm | [Extinction Coefficient]
92,000 | [Microscopy Laser Line]
532 nm or 555 nm | [Flow Cytometry Laser Line]
532 nm or 555 nm | [Spectrally Similar Dyes]
Alexa Fluor® 555, CF® 555, DyLight® 549 |
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