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ODN 1668, a class B CpG ODN (oligodeoxynucleotide), is a TLR-9 agonist. ODN 1668 has strong immune regulatory properties, can enhance the level of antibody IgG2 subtype, promote the immune response of T cells and B cells, and can be used in the study of vaccine adjuvants. In addition, CpG ODN 1668 induces an antimicrobial immune response via a CaTLR9 dependent pathway in groupers. Sequence: 5'-tccatgacgttcctgatgct-3’[1][2][3][4]. | [in vivo]
ODN 1668 (10 nmol ≈ 63.6 μg; injection into the hind footpads; single dose) significantly enhances antibody response in OVA treated mice and increases titers of IgG2a and IgG2b[2].
ODN 1668 (15 μg; intramuscular injection; single dose) improves the antibacterial immunity of Epinephelus laminatus infection, and has the most significant antibacterial activity in liver, spleen and kidney[3].
ODN 1668 (1-5 mg/kg; intraperitoneal/subcutaneous injection; single dose) can cause fever, anorexia and brain inflammation in rats[4].
| Animal Model: | Male Wistar rats (175-200 g)[4] | | Dosage: | 1 and 5 mg/kg | | Administration: | Intraperitoneal and subcutaneous injection, single dose | | Result: | Caused moderate fever and anorexia.
Induced a significant increase of IL-6.
Increased expression of inflammatory genes in the hypothalamus (SOCS3, PGE2, COX-2 and mPGES).
Activated inflammatory transcription factors in the rat brain (STAT3 and NF-IL6). |
| [IC 50]
TLR9 | [References]
[1] Rattanaprukskul K, et al. TLR9 Mediates Periodontal Aging by Fostering Senescence and Inflammaging. J Dent Res. 2022 Dec;101(13):1628-1636. DOI:10.1177/00220345221110108
[2] Lipford GB, et al. CpG-containing synthetic oligonucleotides promote B and cytotoxic T cell responses to protein antigen: a new class of vaccine adjuvants. Eur J Immunol. 1997 Sep;27(9):2340-4. DOI:10.1002/eji.1830270931
[3] Chen X, et al. CpG ODN 1668 as TLR9 agonist mediates humpback grouper (Cromileptes altivelis) antibacterial immune responses. Fish Shellfish Immunol. 2023 Jul;138:108839. DOI:10.1016/j.fsi.2023.108839
[4] Damm J, et al. Intraperitoneal and subcutaneous injections of the TLR9 agonist ODN 1668 in rats: brain inflammatory responses are related to peripheral IL-6 rather than interferons. J Neuroimmunol. 2014 Dec 15;277(1-2):105-17. DOI:10.1016/j.jneuroim.2014.10.007 |
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