| Identification | Back Directory | [Name]
Europium W1024 ITC | [CAS]
1204652-02-7 | [Synonyms]
Europium W1024 ITC
Europate(1-), [[N,N′-[[4-[(4-isothiocyanatophenyl)ethynyl]-2,6-pyridinediyl-κN]bis(methylene)]bis[N-[(carboxy-κO)methyl]glycinato-κN,κO]](4-)]-, sodium (1:1) | [Molecular Formula]
C24H18EuN4O8S.Na | [MOL File]
1204652-02-7.mol | [Molecular Weight]
697.44 |
| Hazard Information | Back Directory | [Description]
Europium W1024 isothiocyanate (ITC) is a lanthanide-based fluorophore with a long emission lifetime and large Stokes shift.1,2 It displays excitation/emission maxima of 340/613 nm, respectively.1 Europium W1024 ITC contains a reactive isothiocyanate handle that reacts with primary amino groups at an alkaline pH. It is stable in the presence of high temperature, high EDTA concentration, or low pH but not in the presence of manganese. Europium W1024 ITC has commonly been conjugated to various molecules and used as a donor fluorophore in TR-FRET where its long emission lifetime provides a longer window of measurement for acceptor fluorescence and reduces the interference of autofluorescence.3,4,5 For Cayman's recommended protein labeling protocol please see the product information sheet.WARNING This product is not for human or veterinary use. | [References]
[1] MATHIS G, BAZIN H. Stable Luminescent Chelates and Macrocyclic Compounds[C]. 1900: 0. DOI: 10.1007/4243_2010_5
[2] MATHIS G. Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer.[J]. Clinical chemistry, 1995, 41 9: 1391-1397.
[3] DEBORAH J. MOSHINSKY . A Widely Applicable, High-Throughput TR-FRET Assay for the Measurement of Kinase Autophosphorylation: VEGFR-2 as a Prototype[J]. SLAS Discovery, 2003, 8 4: Pages 447-452. DOI: 10.1177/1087057103255282
[4] M. SCHEEPSTRA. Identification of an allosteric binding site for RORγt inhibition[J]. Nature Communications, 2015, 6 1. DOI: 10.1038/ncomms9833
[5] DEREK D. POORE Lawrence A W Glenn Hofmann, III . Development of a High-Throughput Cul3-Keap1 Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Assay for Identifying Nrf2 Activators[J]. SLAS Discovery, 2019, 24 2: Pages 175-189. DOI: 10.1177/2472555218807698 |
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