| Chemical Properties | Back Directory | [solubility ]
very poorly soluble in water (0.19 mM = 127 mg/L), good in polar (DMSO, DMF) and chlorinated (DCM, chloroform) organic solvents | [Appearance]
dark colored solid | [InChIKey]
LWGOCTDPEVJNTC-UHFFFAOYSA-N | [SMILES]
[B+3]([F-])([F-])([F-])[F-].C([N+]1=C(C(C)(C)C2=CC=CC=C12)C=CC=CC=C1C(C2=CC=CC=C2N1C)(C)C)CCCCC(=O)ON1C(CCC1=O)=O |
| Hazard Information | Back Directory | [Description]
This Cyanine5 NHS ester, also known as Cy5 NHS ester, is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent to be used in labeling reactions. This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. | [Uses]
Cyanine 5 NHS ester tetrafluoroborate (Compound Cy5) is a cyanine dye that is used to in fluorescence detection of macromolecules. Cyanine 5 NHS ester tetrafluoroborate exhibits an excitation wavelength of 638 nm[1]. | [References]
[1] Kang J, et al., Prevention of H‐aggregates formation in Cy5 labeled macromolecules[J]. International Journal of Polymer Science, 2010, 2010(1): 264781. | [Abs/Em Maxima]
646/662 nm | [Fluoresene quantum yield]
0.2 | [Extinction Coefficient]
250000 | [CF260]
0.03 | [CF280]
0.04 |
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