| Chemical Properties | Back Directory | [solubility ]
good in water, DMF, DMSO | [form ]
Solid | [color ]
Yellow to orange | [Appearance]
dark orange solid | [InChIKey]
SZYLQIQKDQOKDD-UHFFFAOYSA-N | [SMILES]
C(C1C=C(C(=O)ON2C(CCC2=O)=O)C=CC=1C1=C2C=CC(N)=C(S(O)(=O)=O)C2=[O+]C2C(=C(N)C=CC1=2)S([O-])(=O)=O)(=O)O |
| Hazard Information | Back Directory | [Description]
AF488 is a bright and photostable dye, equivalent of Alexa Fluor? 488. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy. This NHS ester is an amine reactive dye, it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules. | [Uses]
AF488 NHS ester is an amine specific fluorescence probe (Em=525 nm). AF488 NHS ester reacts with sulfhydryl groups and amines in aqueous and biological samples then change their chemical structure and fluorescence properties after derivatization[1]. | [References]
[1] Kendall CG, et al. Amine Analysis Using AlexaFluor 488 Succinimidyl Ester and Capillary Electrophoresis with Laser-Induced Fluorescence. J Anal Methods Chem. 2015;2015:368362. DOI:10.1155/2015/368362
[2] Lavoie RA, et al. Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery. Int J Mol Sci. 2019 Apr 8;20(7):1729. DOI:10.3390/ijms20071729 | [Abs/Em Maxima]
495/519 nm | [Fluoresene quantum yield]
0.91 | [Extinction Coefficient]
71800 | [CF260]
0.16 | [CF280]
0.1 |
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