| Hazard Information | Back Directory | [Description]
Cy5 maleimide is a mono-reactive dye containing maleimide group, which selectively couples with thiol groups (for example, with cysteines in peptides and proteins) to yield Cy5 (an analog of Cy5?) labeled conjugates. Sulfo-Cy5 maleimide version is available for the labeling of antibodies and other sensitive proteins. | [Uses]
Cy5 maleimide is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis[1]. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance[2]. | [References]
[1] Maeda K, et al. Cy5 maleimide labelling for sensitive detection of free thiols in native protein extracts: identification of seed proteins targeted by barley thioredoxin h isoforms. Biochem J. 2004 Mar 1;378(Pt 2):497-507. DOI:10.1042/BJ20031634
[2] Ptaszek M. Rational design of fluorophores for in vivo applications. Prog Mol Biol Transl Sci. 2013;113:59-108. DOI:10.1016/B978-0-12-386932-6.00003-X
[3] Shindy, H. A. (2017). Fundamentals in the chemistry of cyanine dyes: A review. Dyes and Pigments, 145, 505–513. doi:10.1016/j.dyepig.2017.06.029 |
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