| Identification | Back Directory | [Name]
Spiro[3H-diazirine-3,6'-cholan]-24'-oic acid, 3'-hydroxy-, 5-hexyn-1-yl ester, (3'β,5'α)- | [CAS]
1485490-47-8 | [Synonyms]
Spiro[3H-diazirine-3,6'-cholan]-24'-oic acid, 3'-hydroxy-, 5-hexyn-1-yl ester, (3'β,5'α)- | [Molecular Formula]
C30H46N2O3 | [MOL File]
1485490-47-8.mol | [Molecular Weight]
482.71 |
| Chemical Properties | Back Directory | [Boiling point ]
577.8±60.0 °C(Predicted) | [density ]
1.22±0.1 g/cm3(Predicted) | [storage temp. ]
-20°C | [solubility ]
Chloroform: 1 mg/ml | [form ]
powder | [pka]
14.96±0.70(Predicted) |
| Hazard Information | Back Directory | [Uses]
PhotoClick Cholesterol is a clickable, photo-reactive sterol probe used in combination with quantitative mass spectrometry to globally map cholesterol-protein interactions directly in living cells. In addition to the UV-activatable cross-linking diazirine group that reacts with proteins, the sterol contains an alkyne group for incorporation of reporter and affinity tags through click-chemistry. | [Biological Activity]
PhotoClick Cholesterol serves as a probe for tracking cholesterol-binding proteins. The copper-catalyzed azide-alkyne cycloaddition is the basis for its probe functionality. The alkyne group is essential for the click chemistry property. Its binding to partners is mediated by photocrosslinking. | [References]
[1] N. CHAUHAN. A PhotoClick cholesterol‐based quantitative proteomics screen for cytoplasmic sterol‐binding proteins in Saccharomyces cerevisiae[J]. Yeast, 2019, 37 1: 15-25. DOI: 10.1002/yea.3448 |
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| Company Name: |
Merck KGaA
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| Tel: |
21-20338288 |
| Website: |
www.sigmaaldrich.cn |
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