| Identification | Back Directory | [Name]
Uridine, 5'-(dihydrogen phosphorothioate) | [CAS]
15548-52-4 | [Synonyms]
5'-UMPS
Uridine, 5'-(dihydrogen phosphorothioate) | [Molecular Formula]
C9H13N2O8PS | [MOL File]
15548-52-4.mol | [Molecular Weight]
340.247 |
| Hazard Information | Back Directory | [Uses]
5'-UMPS (Uridine-5'-O-monophosphorothioate) is a P2Y14 receptor agonist. 5'-UMPS can induce HeLa cell growth slightly[1]. | [Purification Methods]
The Et3N salts are purified by dissolving 4g in 500mL of H2O (adding a drop or two of Et3N if they do not dissolve) and chromatograph by applying to a column (3 x 30cm) of DEAE-Sephadex A-25 and eluting with 1.4L of a linear gradient of Et3NH.HCO3 from 0.05 to 0.55M, pH 7.8 and 4o. The product elutes between 0.20.3M Et3N.HCO3. The pooled fractions are evaporated, and the residue is twice taken up in EtOH and evaporated to dryness to remove the last traces of Et3NH.HCO3. 31P NMR: P is a doublet at -40.81 and -40.33, and P at 7.02ppm, J, 32.96Hz. [Sheu et al. Biochemistry 18 5548 1979.] | [References]
[1] Edyta Gendaszewska-Darmach, et al. Thymidine 5'-O-monophosphorothioate induces HeLa cell migration by activation of the P2Y6 receptor. Purinergic Signal. 2016 Jun;12(2):199-209. DOI:10.1007/s11302-015-9492-1 |
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