| | Identification | Back Directory |  | [Name] 
 Succinimidyl 6-[3-(2-Pyridyldithio)propionamido]hexanoate
 |  | [CAS] 
 158913-22-5
 |  | [Synonyms] 
 SPDP-C6-NHS ester
 N-succinimidyl 6-(3-(2-pyridyldithio)propionamido)hexanoate
 2,5-dioxopyrrolidin-1-yl 6-(3-(pyridin-2-yldisulfaneyl)propanamido)hexanoate
 N-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-3-(2-pyridinyldithio)propanaMide
 Hexanoic acid, 6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]-, 2,5-dioxo-1-pyrrolidinyl ester
 |  | [Molecular Formula] 
 C18H23N3O5S2
 |  | [MDL Number] 
 MFCD00083163
 |  | [MOL File] 
 158913-22-5.mol
 |  | [Molecular Weight] 
 425.52
 | 
 | Chemical Properties | Back Directory |  | [Melting point ] 
 82-84°C
 |  | [density ] 
 1.36±0.1 g/cm3(Predicted)
 |  | [storage temp. ] 
 2-8°C
 |  | [solubility ] 
 DMSO: 100 mg/mL (235.01 mM)
 |  | [form ] 
 Solid
 |  | [pka] 
 15.67±0.46(Predicted)
 |  | [color ] 
 White
 | 
 | Hazard Information | Back Directory |  | [Description] 
 SPDP-C6-NHS ester contains a disulfide bond which is cleavable upon reduction. The NHS ester is reactive toward primary amine and sulfhydryl groups. SPDP-C6-NHS ester is commonly used for bioconjugation and reversible crosslinkings.
 |  | [Chemical Properties] 
 White Solid
 |  | [Uses] 
 A sulfhydryl and amino reactive heterobifunctional protein crosslinking reagent
 |  | [Uses] 
 A sulfhydryl and amino reactive heterobifunctional protein crosslinking reagent.
 |  | [General Description] 
 The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents – see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures.
 |  | [reaction suitability] 
 reagent type: cross-linking reagent
 | 
 | 
                    
                        
                            | Company Name: | Sigma-Aldrich |  
                            | Tel: | 021-61415566 800-8193336 |  
                            | Website: | https://www.sigmaaldrich.cn |  |