| Chemical Properties | Back Directory | [solubility ]
good in water, DMSO | [Appearance]
dark violet solid | [InChIKey]
WTYBXOVGGRVXPB-UHFFFAOYSA-N | [SMILES]
C(C1(C(=[N+](CCCS([O-])(=O)=O)C2=CC=C(S(O)(=O)=O)C=C12)C=CC=CC=C1C(C2=CC(S(O)(=O)=O)=CC=C2N1CCCS(O)(=O)=O)(C)C)C)CCCC(=O)ON1C(CCC1=O)=O |
| Hazard Information | Back Directory | [Description]
AF 647 NHS ester is a bright, far-red fluorescent dye, used for cellular visualization and labeling of antibodies, peptides, proteins, modified oligonucleotides, and other amine-containing molecules.
Due to the lack of significant self-quenching AF 647 dye molecules can be conjugated to proteins at high molar ratios, resulting in the sensitive detection of low-abundance biomolecules potential.
AF 647 NHS ester dye possesses high water-solubility and high fluorescence quantum yield; it is pH-insensitive over a wide molar range. Widely applied for imaging and flow cytometry. | [Uses]
Alexa fluor 647 NHS ester can be used to label Alexa fluor 647 to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules[1]. | [References]
[1] Martin Lehmann, et al. Multicolor Caged dSTORM Resolves the Ultrastructure of Synaptic Vesicles in the Brain. Angew Chem Int Ed Engl. 2015 Nov 2;54(45):13230-5. DOI:10.1002/anie.201505138 | [Abs/Em Maxima]
655/680 nm | [Fluoresene quantum yield]
0.15 | [Extinction Coefficient]
191800 | [CF260]
0.09 | [CF280]
0.08 |
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