| Hazard Information | Back Directory | [Description]
CalFluor 488 Azide is a fluorogenic azide probe that is activated by Cu-catalyzed or metal-free click reaction. This azide probe is not fluorescent until it is reacted with alkynes. CalFluor 488 Azide is a water-soluble probe, and its excitation band is a good match for the 488 nm laser lines (with FITC filter sets). | [Uses]
CalFluor 488 Azide is a water-soluble fluorogenic azide probe. CalFluor 488 Azide is activated by Cu-catalyzed or metal-free click reaction. CalFluor 488 Azide is not fluorescent until it is reacted with alkynes[1][2]. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups. | [References]
[1] Neugebauer ME, et al. Reaction pathway engineering converts a radical hydroxylase into a halogenase. Nat Chem Biol. 2022 Feb;18(2):171-179. DOI:10.1038/s41589-021-00944-x
[2] Hu J, et al. Chiral lipid bilayers are enantioselectively permeable. Nat Chem. 2021 Aug;13(8):786-791. DOI:10.1038/s41557-021-00708-z | [Spectrally Similar Dyes]
FAM, Alexa Fluor® 488, Atto™ 488, CF™ 488A dyes, DyLight™488 |
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