| Identification | Back Directory | [Name]
Sodium (2-Sulfonatoethyl)methanethiosulfonate | [CAS]
184644-83-5 | [Synonyms]
SODIUM (2-SULFONATOETHYL)METHANETHIOSULFONATE
MTSES (Sodium (2-Sulfonatoethyl)methanethiosulfonate) | [Molecular Formula]
C3H9NaO5S3 | [MDL Number]
MFCD00270075 | [MOL File]
184644-83-5.mol | [Molecular Weight]
244.27 |
| Chemical Properties | Back Directory | [Melting point ]
200-203°C | [storage temp. ]
Refrigerator | [solubility ]
DMSO (Slightly), Methanol (Very Slightly), Water (Slightly) | [form ]
Solid | [color ]
White to Off-White | [Stability:]
Moisture Sensitive: Desiccate |
| Hazard Information | Back Directory | [Description]
Methanethiosulfonates (MTS) are sulfhydryl-reactive compounds that form mixed disulfide linkages and are commonly used to study cysteine residues on proteins. Sodium (2-sulfonatoethyl)methanethiosulfonate (MTSES) is a negatively-charged, membrane impermeant MTS. It is highly reactive with ionized thiolates but not with unionized thiols and, therefore, targets sulfhydryl groups accessible from the aqueous medium. MTSES is used to probe the structural and functional properties of native proteins, particularly those associated with membranes, including channels and transporters. In addition, charged MTS compounds like MTSES are combined with cysteine scanning mutagenesis to study non-cysteine residues. | [Chemical Properties]
White Solid | [Uses]
Reacts specifically and rapidly with thiols to form mixed disulfides, Sodium (2-Sulfonatoethyl)methanethiosulfonate can be used to probe the structures of the ACh receptor channel of the GABAA receptor channel and of lactose permease.
| [Uses]
Reacts specifically and rapidly with thiols to form mixed disulfides. Used to probe the structures of the ACh receptor channel of the GABAA receptor channel and of lactose permease. | [References]
[1] RICHARD J LANG Emily L M John R Harvey. Sodium (2-sulfonatoethyl) methanethiosulfonate prevents S-nitroso-L-cysteine activation of Ca2+-activated K+ (BKCa) channels in myocytes of the guinea-pig taenia caeca[J]. British Journal of Pharmacology, 2009, 139 6: 1153-1163. DOI: 10.1038/sj.bjp.0705349
[2] R A LI. Pore residues critical for mu-CTX binding to rat skeletal muscle Na+ channels revealed by cysteine mutagenesis.[J]. Biophysical journal, 1997, 73 4: 1874-1884. DOI: 10.1016/s0006-3495(97)78218-3
[3] LAN GUAN H R K. Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins[J]. Nature Protocols, 2007, 2 8: 2012-2017. DOI: 10.1038/nprot.2007.275
[4] ANITA M ENGH Merritt M. Cysteine accessibility in ClC-0 supports conservation of the ClC intracellular vestibule.[J]. Journal of General Physiology, 2005, 125 6: 601-617. DOI: 10.1085/jgp.200509258
[5] XUEHONG LIU. Variable reactivity of an engineered cysteine at position 338 in cystic fibrosis transmembrane conductance regulator reflects different chemical states of the thiol.[J]. The Journal of Biological Chemistry, 2006, 281 12: 8275-8285. DOI: 10.1074/jbc.m512458200 |
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| Company Name: |
Energy Chemical
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021-58432009 400-005-6266 |
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http://www.energy-chemical.com |
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