| Identification | Back Directory | [Name]
Alexa Fluor 350 | [CAS]
200554-19-4 | [Synonyms]
BF 350, SE
Alexa Fluor 350
AF350 NHS Ester
Alexa Fluor 350 NHS ester
2H-1-Benzopyran-3-acetic acid, 7-amino-4-methyl-2-oxo-6-sulfo-, 3-(2,5-dioxo-1-pyrrolidinyl) ester | [Molecular Formula]
C16H14N2O9S | [MOL File]
200554-19-4.mol | [Molecular Weight]
410.35 |
| Chemical Properties | Back Directory | [density ]
1.74±0.1 g/cm3(Predicted) | [solubility ]
Soluble in water, dimethyl sulfoxide, methanol
| [pka]
0.46±0.20 Most Basic Temperature: 25°C | [color ]
Pale yellow precipitate2 or yellow/ orange solid | [Appearance]
Gray yellow solid | [ex/em]
346/445 nm | [ε(extinction coefficient)]
19,000 | [λmax]
346nm (Buffer pH 7); 353nm (MeOH) | [Φ(quantum yield)]
0.73 | [Biological Applications]
Analyzing peptides; detecting mutations; detecting/identifying nucleic acids; detecting/isolating/purifying proteins; investigating location of phosphatidylinositol,bisphosphate (PI(,)P) in cell membranes; colloidal diagnostic devices; multiplex genetic analysis; as nucleic acid hybridization probe; useful for synthesizing nucleic acid,, peptides, | [Major Application]
Poly(vinyl alcohol)coated microfluidic devices; selfassembled monolayers; silica particles/beads |
| Hazard Information | Back Directory | [Chemical Properties]
Appearance: Grayish yellow solid
ex/em: 346/445 nm
Extinction coefficient (ε): 19,000
Quantum yield (Φ): 0.73 | [Uses]
BP Fluor 350 NHS ester is an amine reactive, water soluble, blue emitting dye used to specifically and efficiently modify primary amines at pH 7-9 to form stable covalent amide bonds[1]. | [Definition]
ChEBI:Alexa Fluor 350 is a chromenone, a pyrrolidinone and an organosulfonic acid. It has a role as a fluorochrome. | [References]
[1] Khoury JT, et al. Pulsed fluorescence measurements of trapped molecular ions with zero background detection. J Am Soc Mass Spectrom. 2002 Jun;13(6):696-708. DOI:10.1016/S1044-0305(02)00364-1 |
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