| Identification | Back Directory | [Name]
4,5-DIAMINOFLUORESCEIN | [CAS]
205391-01-1 | [Synonyms]
DAF-2
5-Diaminofluorescein
4,5-DIAMINOFLUORESCEIN
5,6-DIAMINOFLUORESCEIN
DAF-2;4;5-DIAMINOFLUORESCEIN
4,5-Diaminofluorescein, DAF-2
4,5-Diaminofluorescein-Isopropanol adduct (1:2)
2-(3,6-DIHYDROXY-9H-XANTHEN-9-YL)-4,5-DIAMINO-BENZOIC ACID
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5,6-diamino-3',6'-dihydroxy- | [Molecular Formula]
C20H14N2O5 | [MDL Number]
MFCD05865100 | [MOL File]
205391-01-1.mol | [Molecular Weight]
362.34 |
| Chemical Properties | Back Directory | [storage temp. ]
2-8°C | [form ]
powder | [color ]
Brown to black | [InChI]
1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2 | [InChIKey]
LTYUPYUWXRTNFQ-UHFFFAOYSA-N | [SMILES]
Nc1cc2c(cc1N)C(=O)OC32c4c(cc(cc4)O)Oc5c3ccc(c5)O |
| Hazard Information | Back Directory | [Description]
DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively. At neutral pH the detection limit for NO is 2-5 nM. | [Uses]
DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. | [Uses]
Fluorescent detector of nitric oxide in culture medium, tissue sections and biopsy cells. | [References]
[1] H KOJIMA. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore.[J]. Chemical & pharmaceutical bulletin, 1998, 46 2: 373-375. DOI: 10.1248/cpb.46.373
[2] NAOKI NAKATSUBO. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins[J]. FEBS Letters, 1998, 427 2: 263-266. DOI: 10.1016/s0014-5793(98)00440-2 |
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