Identification | Back Directory | [Name]
4,5-DIAMINOFLUORESCEIN | [CAS]
205391-01-1 | [Synonyms]
DAF-2 5-Diaminofluorescein 4,5-DIAMINOFLUORESCEIN 5,6-DIAMINOFLUORESCEIN DAF-2;4;5-DIAMINOFLUORESCEIN 4,5-Diaminofluorescein, DAF-2 4,5-Diaminofluorescein-Isopropanol adduct (1:2) 2-(3,6-DIHYDROXY-9H-XANTHEN-9-YL)-4,5-DIAMINO-BENZOIC ACID Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 5,6-diamino-3',6'-dihydroxy- | [Molecular Formula]
C20H14N2O5 | [MDL Number]
MFCD05865100 | [MOL File]
205391-01-1.mol | [Molecular Weight]
362.34 |
Hazard Information | Back Directory | [Description]
DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively. At neutral pH the detection limit for NO is 2-5 nM. | [Uses]
DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. | [Uses]
Fluorescent detector of nitric oxide in culture medium, tissue sections and biopsy cells. | [References]
[1] H KOJIMA. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore.[J]. Chemical & pharmaceutical bulletin, 1998, 46 2: 373-375. DOI: 10.1248/cpb.46.373 [2] NAOKI NAKATSUBO. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins[J]. FEBS Letters, 1998, 427 2: 263-266. DOI: 10.1016/s0014-5793(98)00440-2 |
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