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BODIPY FL-EDA is a widely used fluorescent dye for quantitative analysis of nucleotides. BODIPY FL-EDA is an aliphatic amine analog that can react with aldehydes and ketones. BODIPY FL-EDA can be used to detect both modified and unmodified deoxynucleotides and to determine DNA damage and genomic DNA methylation through capillary electrophoresis with laser-induced fluorescence (CE-LIF). Additionally, it can be used for quantifying intracellular ATP levels. The excitation wavelength is 500 nm, and the emission wavelength is 510 nm[1][2][3]. | [References]
[1] Cornelius M, et al. Detection and separation of nucleoside-5'-monophosphates of DNA by conjugation with the fluorescent dye BODIPY and capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis. 2005 Jun;26(13):2591-8. DOI:10.1002/elps.200410405
[2] Zinellu A, et al. Capillary electrophoresis with laser-induced fluorescence detection for ATP quantification in spermatozoa and oocytes. Anal Bioanal Chem. 2010 Nov;398(5):2109-16. DOI:10.1007/s00216-010-4186-6
[3] Cornelius MG, et al. RNA analysis by MEKC with LIF detection. Electrophoresis. 2007 Nov;28(21):3901-7. DOI:10.1002/elps.200700127 |
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