| Identification | Back Directory | [Name]
5-FLUOROOROTIC ACID HYDRATE 98 | [CAS]
220141-70-8 | [Synonyms]
Fluoroorotic Monohydrate
5-FLUOROOROTIC ACID HYDRATE 98
5-FOA (5-Fluoroorotic acid), Monohydrate
5-Fluoroorotic Acid Monohydrate (Ultra Pure)
5-fluoro-2,4-dioxo-1H-pyrimidine-6-carboxylic acid
5-Fluoro-2,6-dioxo-1,2,3,6-tetrahydropyriMidine-4-carboxylic acid hydrate
5-Fluoroorotic Acid Monohydrate (5-FOA Monohydrate) for molecular biology, 98% | [EINECS(EC#)]
678-852-3 | [Molecular Formula]
C5H5FN2O5 | [MDL Number]
MFCD00150658 | [MOL File]
220141-70-8.mol | [Molecular Weight]
192.102 |
| Chemical Properties | Back Directory | [Melting point ]
278 °C (dec.)(lit.)
| [storage temp. ]
Inert atmosphere,Store in freezer, under -20°C | [solubility ]
Aqueous Base (Heated), DMSO (Sparingly, Heated), Methanol (Sparingly), Water (Sl | [form ]
Solid | [color ]
Must be White | [Water Solubility ]
Soluble in water, dimethyl sulfoxide, 50mg in 1ml (1:1 ammonium hydroxide: water) with gentle heating. | [Merck ]
14,4176 | [BRN ]
181933 |
| Hazard Information | Back Directory | [Description]
5-fluoro Orotic acid (5-FOA) is used to detect URA3 gene expression in yeast molecular genetic constructs. Yeast with an active URA3 gene, which encodes orotine-5''-monophosphate decarboxylase, converts 5-FOA to fluorodeoxyuridine, which is toxic to cells. Resistant strains of yeast that carry a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil. | [Uses]
5-Fluoroorotic Acid, Monohydrate (5-FOA) has become a valuable tool for research in the field of molecular genetics and has been employed in the selection of resistant strains of Saccharomyces cerevisiae that possess a mutant URA3 gene which renders them orotidine-5í-phosphate decarboxylation. | [References]
[1] F WINSTON. Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae.[J]. Genetics, 1984, 107 2: 179-197. DOI: 10.1093/genetics/107.2.179 |
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