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Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc).1,2 It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h , which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols.3 Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10.2,3 BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.1,2,4 | [References]
1. Bertrand, L., Parent, S., Caron, M., et al. The BRET2/arrestin assay in stable recombinant cells: A platform to screen for compounds that interact with G protein-couple receptors (GPCRS) J. Recept. Signal Transduct. Res. 22(1-4),533-541(2002).
2. Galés, C., Rebois, R.V., Hogue, M., et al. Real-time monitoring of receptor and G-protein interactions in living cells Nat. Methods 2(3),177-184(2005).
3. Pfleger, K.D., and Eidne, K.A. Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET) Nat. Methods 3(3),165-174(2006).
4. Huang, Q., Acha, V., Yow, R., et al. Bioluminescence measurements in mice using a skin window J. Biomed. Opt. 12(5):054012,(2007). |
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