| Identification | Back Directory | [Name]
AF532 carboxylic acid | [CAS]
2791287-09-5 | [Synonyms]
AF532 acid
AF532 COOH
axlea 532 acid
AF532 carboxylic acid
5-(4-carboxyphenyl)-2,3,3,7,7,8-hexamethyl-12-sulfo-2,3,7,8-tetrahydro-1H-pyrano[3,2-f:5,6-f']diindol-9-ium-10-sulfonate | [Molecular Formula]
C30H30N2O9S2 | [MOL File]
2791287-09-5.mol | [Molecular Weight]
626.7 |
| Hazard Information | Back Directory | [Description]
AF 532 is a bright, photostable, and hydrophilic fluorophore emitting in the yellow-green channel, an alternative to HEX, JOE, and SIMA. The dye is useful in super-resolution microscopy, particularly in stochastic optical reconstruction microscopy (STORM), as an activator in nSTORM and a reporter in dSTORM.
AF 532 carboxylic acid is a non-reactive form of AF 532 dye that can be used as a reference standard in experiments involving AF 532 dye conjugates. Besides, the carboxylic group can react with hydrazines, hydroxylamines, and amines using carbodiimides such as EDAC. | [Abs/Em Maxima]
524/547 nm | [Fluoresene quantum yield]
0.6 | [Extinction Coefficient]
80500 | [CF260]
0.17 | [CF280]
0.11 |
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