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5-Ph-IAA-AM is an eggshell-permeable 5-Ph-IAA (HY-134653) analog which contains an acetoxymethyl (AM) group. 5-Ph-IAA-AM affords an enhanced protein degradation in laid embryos. 5-Ph-IAA-AM can be used to disclosure the roles of proteins in C. elegans, in particular those that are involved in embryogenesis and development, through temporally controlled protein degradation[1][2][3]. | [in vivo]
5-Ph-IAA-AM (50 μM, 60 min) induces better protein degradation in the C. elegans embryos than does 5-Ph-IAA (50 μM, 60 min)[1].
5-Ph-IAA-AM (50 mM, injection, a single dose for 0-50 min)-injected embryos reproducibly fails to form ventral furrows or undergo any other gastrulation processes, which is consistent with loss of myosin function in early Drosophila embryo[2][3].
| Animal Model: | early Drosophila embryo[2][3] | | Dosage: | 50 mM | | Administration: | 0-50 min, injection | | Result: | The fluorescence of 5-Ph-IAA-AM-injected embryos was found to drop to its minimum within 30-50 min after the injection. |
| [References]
[1] Negishi T, et al. The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans. Genetics. 2022 Feb 4;220(2):iyab218. DOI:10.1093/genetics/iyab218
[2] Goldner AN, et al. Evidence that tissue recoil in the early Drosophila embryo is a passive not active process[J]. Mol Biol Cell. 2023 Sep 1;34(10):br16. DOI:10.1091/mbc.E22-09-0409
[3] Eijlers P, et al. A nematode-specific ribonucleoprotein complex mediates interactions between the major nematode spliced leader snRNP and its target pre-mRNAs. Nucleic Acids Res. 2024 Jul 8;52(12):7245-7260. DOI:10.1093/nar/gkae321 |
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