| Identification | Back Directory | [Name]
QSY 21 CARBOXYLIC ACID,
SUCCINIMIDYL ESTER | [CAS]
304014-13-9 | [Synonyms]
Qsy
21
QSY21 SE
Qsy
21nhs
QSY21 NHS ester
QSY 21 CARBOXYLIC ACID,
SUCCINIMIDYL ESTER
Xanthylium, 3,6-bis(2,3-dihydro-1h-indol-
1-yl)-9-[2-[[4-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]-
1-piperidinyl]sulfonyl]phenyl]-, Chloride | [Molecular Formula]
C45H39ClN4O7S | [MOL File]
304014-13-9.mol | [Molecular Weight]
815.34 |
| Chemical Properties | Back Directory | [Melting point ]
>200℃ | [solubility ]
Soluble in dimethyl sulfoxide, methanol | [form ]
Solid | [color ]
Purple solid | [Appearance]
dark purple powder | [λmax]
661 nm | [Biological Applications]
Amplification assays; hybridization assays; litigation assays; ligand assays; detecting analytes,enzymes,optical microcavities,protein– DNA interactions; imaging apoptosis,b-lactamase activity; FRET probes |
| Hazard Information | Back Directory | [Description]
DusQ® 21 is a dark quencher used as an acceptor in FRET applications, it has absorption maximum 656 nm, and can be used in multiplex assays due to the lack of secondary fluorescence. It is a quencher of choice for pairing with fluorescent dyes that emit in the red and near-infrared part of the range. DusQ 21 NHS ester derivative is used for labeling with the primary amines of amine-containing molecules. It can be used for quenching of many fluorophores, including Cyanine5, Cyanine5.5, AF 647. | [Uses]
QSY 21 NHS, a dark quencher is an efficient energy transfer acceptor of the far red and NIR fluorescent probes. QSY 21 NHS works in
the wavelength range of 540-750 nm, and is frequently used in FRET applications. QSY 21 NHS does not emit fluorescence in normal conditions. NHS esters can be used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules[1]. | [References]
[1] Kabelác M, et al. A comparative study of the binding of QSY 21 and Rhodamine 6G fluorescence probes to DNA: structure and dynamics. Phys Chem Chem Phys. 2010 Sep 7;12(33):9677-84. DOI:10.1039/c004020g | [Abs/Em Maxima]
656 nm | [Extinction Coefficient]
72800 |
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