ChemicalBook--->CAS DataBase List--->3326-35-0

3326-35-0

3326-35-0 Structure

3326-35-0 Structure
IdentificationBack Directory
[Name]

4-NITROFLUORESCEIN
[CAS]

3326-35-0
[Synonyms]

4-NITROFLUORESCEIN
4-NITROFLUOROESCEIN
5-Nitrofluorescein (isomer I)
3',6'-dihydroxy-6-nitrospiro[2-benzofuran-3,9'-xanthene]-1-one
3',6'-dihydroxy-6-nitro-spiro[2-benzofuran-3,9'-xanthene]-1-one
3',6'-dihydroxy-6-nitro-spiro[isobenzofuran-3,9'-xanthene]-1-one
5-NITRO-3',6'-DIHYDROXYSPIRO[ISOBENZOFURAN-1(3H),9'-(9H)XANTHEN]-3-ONE
3',6'-Dihydroxy-5-nitrospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
5-Nitro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3-one
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,3',6'-dihydroxy-5-nitro-
[Molecular Formula]

C20H11NO7
[MDL Number]

MFCD00135108
[MOL File]

3326-35-0.mol
[Molecular Weight]

377.3
Chemical PropertiesBack Directory
[Appearance]

Orange Cyrstalline Solid
[Melting point ]

>300°C
[Boiling point ]

692.0±55.0 °C(Predicted)
[density ]

1.72±0.1 g/cm3(Predicted)
[storage temp. ]

2-8°C
[solubility ]

almost transparency in NaOH100g/L
[form ]

powder to crystal
[pka]

9.29±0.20(Predicted)
[color ]

Light yellow to Brown
[Stability:]

Light and Moisture Sensitive
[InChI]

InChI=1S/C20H11NO7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)20(15)14-4-1-10(21(25)26)7-13(14)19(24)28-20/h1-9,22-23H
[InChIKey]

UACQOMKZFGNRBL-UHFFFAOYSA-N
[SMILES]

C12(C3=C(C=C(O)C=C3)OC3=C1C=CC(O)=C3)C1=C(C=C([N+]([O-])=O)C=C1)C(=O)O2
[EPA Substance Registry System]

Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-nitro- (3326-35-0)
Hazard InformationBack Directory
[Chemical Properties]

Orange Cyrstalline Solid
[Uses]

Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens
[Synthesis]

4-Nitrofluorescein was prepared as follows: 8.6 g (18.7 mmol) of ethyl 4-nitrofluorescein triacetate was dissolved in 87 mL of 10% sodium hydroxide solution and 432 mL of methanol and gently heated. The precipitate was filtered and dissolved in 500 mL of
Safety DataBack Directory
[Symbol(GHS) ]

Exclamation Mark (GHS07)
GHS07
[Signal word ]

Warning
[Hazard statements ]

H302-H315-H319-H335
[Precautionary statements ]

P280-P305+P351+P338
[TSCA ]

TSCA listed
[HS Code ]

2932.99.7000
Raw materials And Preparation ProductsBack Directory
[Preparation Products]

Fluorescein isothiocyanate
Spectrum DetailBack Directory
[Spectrum Detail]

4-Nitrofluorescein(3326-35-0)MS
4-Nitrofluorescein(3326-35-0)1HNMR
4-Nitrofluorescein(3326-35-0)IR1
4-Nitrofluorescein(3326-35-0)IR2
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