| Identification | Back Directory | [Name]
PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming | [CAS]
37288-19-0 | [Synonyms]
PC-PLC
phosphatidylinositol diacylglycerol-lyase
PI-PLC, 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus lyophilized powder, >=1,000 units/mg protein (Lowry) | [EINECS(EC#)]
232-638-2 | [MDL Number]
MFCD00131896 |
| Chemical Properties | Back Directory | [storage temp. ]
-20°C | [form ]
lyophilized powder | [biological source]
Bacillus sp. (Bacillus cereus) | [Specific Activity]
≥1,000units/mg protein (Lowry) |
| Hazard Information | Back Directory | [Uses]
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells. | [Uses]
Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:
- in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)
- to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membrane
- to cleave immunolabeled HeLa cells
| [General Description]
Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI. | [Biochem/physiol Actions]
Used for the release of GPI anchored proteins from the membrane. |
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