| Identification | Back Directory | [Name]
Proteinase K | [CAS]
39450-01-6 | [Synonyms]
PROK
20mg
40U/MG
CHLOROM
3.4.21.14
Protease K
KProteinas
PROTEINASE K
EC 3.4.21.14
EC 3.4.21.64
KProteinase K
IUB: 3.4.21.14
IUB: 3.4.21.64
ENDOPEPTIDASE K
Einecs 254-457-8
RecoMbinant Proteinase K
PROTEINASE K, IMMOBILIZED
PROTEINASE K, LYOPHILIZED
ProteinaseK(E.C.3.4.21.14)
Tritirachium album proteinase K
PROTEINASE K TRITIRACHIUM ALBUM
Proteinase K Solution, 20 mg/ml
BOTTLE 1L SMALL BROWN GLASS DIN45
PROTEINASE K FROM TRITRACHIUM ALBUM
proteinase k for tritirachium album
PROTEINASE K FROM TRITIRACHIUM ALBUM
Proteinase K from tritirachium abbum
Proteinase, Tritirachium album serine
NUTRIENT +BLUE +TTC PETRI 90MM (6X20)
ProteinaseK,PowderAndSolutionAvailable
ProteinaseK,fromTritirachiumalbumLimber
Proteinase K, tritirachium album serine
CT-SMAC (E.COLI 0:157) PETRI 90MM (6X20)
PROTEINASEK,LYOPHILIZEDPOWDER,BIOTECHGRADE
Proteinase K for tritirachium album serine.
PROTEINASE K SOLUTION IN 40%*(V/V) GLYCE ROL
Proteinase K from Tritirachium album lyophil.
PROTEINASE K FROM TRITIRACHIUM ALBUM 290 U/MG
Proteinase Ksolution,from Tritirachium album
PROTEINASE K FROM TRITIRACHIUM ALBUM, >500 U/MG*
Proteinase K from Tritirachium album ca. 8 DMC-U/mg
Proteinase K from Tritirachium album,Endopeptidase K
PROTEINASE K FROM TRITIRACHIUM ALBUM*MOL ECULAR BIOL
PROTEINASE K FOR TRITIRACHIUM ALBUM, FOR MOL. BIOLOGY
PROTEINASE K FR. TRITIRACHIUMALBUM CA.8 DCM-U/MG LYOPH.
Proteinase K froM TritirachiuM albuM ca. 8 DMC-U/Mg lyophil.
Endopeptidase K
Prok
Protease K
Tritirachium album proteinase K
Proteinase K from Tritirachium album [EC 3.4.21.64] from Tritirachium album
Proteinase K from Tritirachium album Vetec(TM) reagent grade, powder, >=30 units/mg protein
PROTEINASEK TRITIRACHIUM ALBUM FOR MOLECULAR BIOLOGY, BUFFERED AQUEOUS GLYCEROL SOLUTION, DNASE, NICKASE AND RNASE NONE | [EINECS(EC#)]
254-457-8 | [Molecular Formula]
n.a. | [MDL Number]
MFCD00132129 | [MOL File]
39450-01-6.mol | [Molecular Weight]
626.505 |
| Chemical Properties | Back Directory | [Appearance]
Powder beige | [storage temp. ]
2-8°C
| [solubility ]
H2O: 10 mg/mL, slightly hazy, brown-yellow
| [form ]
powder
| [color ]
white
| [PH]
pH7.5～12 | [Water Solubility ]
Miscible with water. | [InChIKey]
CDWGKFQEIBBJOF-BIZWTRPUNA-N | [SMILES]
N12[C@](OP(O)(OC3=CC=C([N+]([O-])=O)C=C3)=O)([C@H]([C@H](O)C)C1=O)[C@](C)(OC1=CC=CC=C1)C(OC1=CC=CC=C1)=C2C(OC)=O |&1:1,16,17,22,r| | [CAS DataBase Reference]
39450-01-6 |
| Hazard Information | Back Directory | [Chemical Properties]
Powder beige | [Description]
Proteinase K is a proteolytic enzyme (a serine protease) naturally found in the mold Tritirachium album. In solution, it is stable over a pH range of 4.0–12.5 with an optimum of pH 8.0, and a temperature range of 25°C–65°C. The enzyme has two binding sites for Ca++, though in the absence of this divalent cation some catalytic activity is retained. Maximum proteinase K activity is observed with the inclusion of 1 mM Ca++ in the reaction buffer. Proteinase K digestion is routinely performed at 50°C, and occasionally in the presence of EDTA to inhibit labile, Mg++-dependent nucleases. Proteinase K is prepared commonly as a 20 mg/mL stock solution in sterile water (stable for 1 year at ?20°C) or in a solution of 50 mM Tris, pH 8.0, 1 mM CaCl2 (stable for months at 4°C). It is generally used at a working concentration of up to 50 μg/mL in any of a number of buffer formulations, including those that contain as much as 0.5% SDS[1].
| [Uses]
A stable and highly reactive serine protease; used for protein and nucleic acid isolation.Proteinase K is used in the purification of RNA and DNA from tissues or cell lines. It finds application in protein modification, determination of enzyme localization and inactivation of RNases/DNases during nucleic acid extraction. It is also used in mitochondria isolation and removal of endotoxins bound to cationic proteins like lysozyme and ribonuclease A. Further, it is involved in the treatment of paraffin embedded tissue sections in order to expose antigen binding sites for antibody labeling. | [Definition]
Proteinase K cleaves peptide bonds next to the carboxyl-terminal of aromatic amino acids, hydrophobic amino acids and sulfuric amino acids, within the polypeptide chain. Hence Proteinase K can act as an endoprotease on a wide range of protein substrates. Because of its proteolytic preferences, it is a member of the subtilisin group of serine proteases. The K in Proteinase K refers to its ability to digest Keratin protein found in hairs, nails and hooves. Proteinase K is an essential reagent in molecular biology labs because it is useful in isolating DNA and RNA molecules by degrading and inactivating proteins. Fungus Tritirachium album Limber naturally produces proteinase K.
| [General Description]
Proteinase K, an extracellular endopeptidase is synthesized by the mold, Tritirachium album Limber. Proteinase K belongs to a new subfamily of the subtilisins. It is a 277 amino acid protein and is characterized with an unhydrolyzed protein chain and autolyzed polypeptide chains. | [Biochem/physiol Actions]
Proteinase K catalyzes the hydrolysis of keratin. | [References]
[1] Farrell, R. “Creating a Ribonuclease-Free Environment.”RNA Methodologies (Sixth Edition) 2023: 51-70.
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