| Identification | Back Directory | [Name]
MUNG BEAN NUCLEASE | [CAS]
54576-84-0 | [Synonyms]
NUCLEASE
BENZONASE
NUCLEASE R
Nuclease P
NUCLEASE S1
NUCLEASE P1
S1 NUCLEASE
EC 3.1.30.1
EC 3.1.30.2
BENZONASE(R)
ENDONUCLEASE
IUB: 3.1.30.1
NUCLEASE-GAMMA
BENZON NUCLEASE
endonuclease p1
MUNG BEAN NUCLEASE
BENZONASE NUCLEASE
NUCLEASE, MUNG BEAN
BENZONASE(R) NUCLEASE
NUCLEASE S1 (ASPERGILLUS ORYZAE)
Nuclease P1, Penicillium citrinum
NUCLEASE P1 FROM PENCILLIUM CITRINUM
ENDONUCLEASE S1 (ASPERGILLUS ORYZAE)
Nuclease P1 from Penicillium citrinum
nuclease P-1 from penicillium citrinum
nuclease P(1) from penicillium citrinum
Native Penicillium citrinum Nuclease P1
Nuclease P1 from Penicillium citrinum,Endonuclease P1
NUCLEASE P(1) FROM PENICILLIUM CITRINUM, LYOPH., ~1200 U/MG* | [EINECS(EC#)]
253-443-9 | [Molecular Formula]
NULL | [MDL Number]
MFCD00131010 |
| Chemical Properties | Back Directory | [Definition]
An enzyme which digests only
single-stranded nucleic acids.
| [storage temp. ]
2-8°C
| [form ]
lyophilized powder
| [color ]
White to light yellow | [biological source]
Penicillium citrinum | [Specific Activity]
≥200units/mg protein (E1%/280, 3μ-5μ-Phosphodiesterase) | [CAS DataBase Reference]
54576-84-0 |
| Hazard Information | Back Directory | [Uses]
The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8. | [General Description]
Nuclease P1 is one of the most commonly known single-strand specific nucleases in molecular biology; Nuclease P1 is a single stranded specific endoduclease (of ssDNA or ssRNA). Nuclease P1 can also cleave single-stranded regions in double-stranded nucleic acids.
Nuclease P1 from Penicillium citrinum is a zinc-dependent endonuclease that exhibits increased activity in the presence of low concentrations of urea. Nuclease P1 selective activity has found useful applications in studies on nucleic acid structure. | [Biochem/physiol Actions]
Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. It does not appreciably degrade double-stranded nucleic acids, especially in the presence of more than 400 mM sodium chloride at pH 6.0. | [Synthesis]
There are three main production methods for nuclease P1: solid-state fermentation, deep liquid fermentation and immobilized liquid fermentation. Solid-state fermentation (solid-state fermentation, SSF) operation is simple, low cost, the use of bra |
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