| Identification | Back Directory | [Name]
FPG PROTEIN | [CAS]
78783-53-6 | [Synonyms]
fpg protein from escherichia coli
DNA-formamidopyrimidine glycosylase
Fpg Protein from Escherichia coli, Recombinant
Fpg Protein from Escherichia coli >=90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, >20,000 units/mg protein | [MDL Number]
MFCD02264758 |
| Chemical Properties | Back Directory | [storage temp. ]
-20°C | [form ]
buffered aqueous glycerol solution | [biological source]
Escherichia coli | [Specific Activity]
>20,000units/mg protein | [Major Application]
genomic analysis |
| Hazard Information | Back Directory | [Uses]
Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay. | [General Description]
Fpg contains two domains separated by a flexible hinge. | [Biochem/physiol Actions]
Fpg is a key enzyme in the DNA base excision repair pathway (BER). It catalyses the excision of a broad spectrum of modified purines. Fpg has both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose leaving both 5′- and 3′-phosphorylated ends in the DNA. The zinc finger motif at its C-terminus is responsible for the DNA binding and AP-lyase activity. In addition, its N-terminal proline acts as a nucleophile to produce a Schiff base intermediate that is essential for enzyme action. |
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| Company Name: |
Sigma-Aldrich
|
| Telephone: |
021-61415566 800-8193336 |
| Website: |
https://www.sigmaaldrich.cn |
| Company Name: |
Energy Chemical
|
| Telephone: |
021-58432009 400-005-6266 |
| Website: |
http://www.energy-chemical.com |
|