| Identification | Back Directory | [Name]
CI 50200 | [CAS]
81-93-6 | [Synonyms]
nsc9855
CI 50200
CI NO 50200
schultzno.958
PHENOSAFRANIN
PHENOSAFRANINE
Phenosafeanine
phenosofranine
phenosofrinine
safrininbextra
Safranine B Extra
phenosafranine,chloride
PHENOSAFRANINE FOR MICROSCOPY
3,7-diamino-5-phenyl-phenaziniuchloride
3,7-DIAMINO-5-PHENYLPHENAZINIUM CHLORIDE
Phenazinium,3,7-diamino-5-phenyl-,chloride
2,8-DiaMino-10-phenyl-phenaziniuM Chloride
3,7-Diamino-5-phenylphenazin-5-ium chloride | [EINECS(EC#)]
201-387-0 | [Molecular Formula]
C18H15ClN4 | [MDL Number]
MFCD00036335 | [MOL File]
81-93-6.mol | [Molecular Weight]
322.79 |
| Chemical Properties | Back Directory | [Appearance]
green crystalline powder | [Melting point ]
300 °C
| [Boiling point ]
478.49°C (rough estimate) | [density ]
1.1738 (rough estimate) | [refractive index ]
1.6110 (estimate) | [storage temp. ]
room temp | [Colour Index ]
50200 | [form ]
Solid | [color ]
Green to dark green | [λmax]
519 nm | [Merck ]
13,7333 | [BRN ]
3642082 | [CAS DataBase Reference]
81-93-6 |
| Hazard Information | Back Directory | [Chemical Properties]
green crystalline powder | [Uses]
A bacterial stain used in microscopy. | [Definition]
ChEBI: An organic chloride salt having 3,7-diamino-5-phenylphenazin-5-ium as the counterion. It is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms. | [Uses]
Biological stain. | [Biochem/physiol Actions]
Phenosafranin (PSF) is a red dye released from cells with intact plasma membrane. PSF at lower concentration is used to stain mitochondria supravitally. | [Staining Procedures]
Phenosafranin is used to distinguish living from dead cells. A 0.1 % aqueous solution, it is taken up by dead or abnormal cells immediately, while living cells remain unstained for at least 30 min. | [Purification Methods]
Crystallise the chloride from dilute HCl. It has UV with at 530nm in H2O. The picrate decomposes on heating and has a solubility of 0.0048% in H2O at 18o. [Beilstein 23 H 395, Beilstein 25 H 394, 25 I 654, 25 II 338, 25 III/IV 3050.] |
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| Company Name: |
J & K SCIENTIFIC LTD.
|
| Telephone: |
18210857532 18210857532 |
| Website: |
https://www.jkchemical.com |
| Company Name: |
Spectrum Chemical Manufacturing Corp.
|
| Telephone: |
021-021-021-67601398-809-809-809 15221380277 |
| Website: |
www.spectrumchemical.com/oa_html/index.jsp?minisite=10020&respid=22372&language=us |
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