| Identification | Back Directory | [Name]
METHYL GREEN | [CAS]
82-94-0 | [Synonyms]
CI 42585
CI NO 42585
CI NO 42590
PARIS GREEN
LIGHT GREEN
METHYL GREEN
DOUBLE GREEN
BASIC BLUE 20
DOUBLE GREEN SF
CI BASIC BLUE 20
C.I. 42585 or 42590
Methyl Green zinc ch
GLYCEROL /METHYL GREEN
Methyl Green Dichloride
HEPTAMETHYL-P-ROSANILINE CHLORIDE
METHYL GREEN ZINC CHLORIDE DOUBLE SALT
n,n-trimethyl-n-1-ylidene]methyl]-dichloride
[4-[(4-dimethylaminophenyl)-(4-dimethyliminiocyclohexa-2,5-dien-1-ylidene)methyl]phenyl]-trimethyl-ammonium dichloride
4-{[4-(DiMethylaMino)phenyl][4-(diMethyliMinio)-2,5-cyclohexadien-1-ylidene]Methyl}-N,N,N-triMethylaniliniuM dichloride
4-[[4-(Dimethylamino)phenyl][4-(dimethyliminio)cyclohexa-2,5-dien-1-ylidene]methyl]-N,N,N-trimethylanilinium dichloride
Benzenaminium, 4-4-(dimethylamino)phenyl4-(dimethyliminio)-2,5-cyclohexadien-1-ylidenemethyl-N,N,N-trimethyl-, dichloride
[4-[(4-dimethylaminophenyl)-(4-dimethylazaniumylidenecyclohexa-2,5-dien-1-ylidene)methyl]phenyl]-trimethylazanium dichloride
[4-[(4-dimethylaminophenyl)-(4-dimethylazaniumylidenecyclohexa-2,5-dien-1-ylidene)methyl]phenyl]-trimethyl-azanium dichloride | [EINECS(EC#)]
201-447-6 | [Molecular Formula]
C26H33Cl2N3 | [MDL Number]
MFCD00011879 | [MOL File]
82-94-0.mol | [Molecular Weight]
458.47 |
| Chemical Properties | Back Directory | [storage temp. ]
2-8°C, sealed storage, away from moisture and light | [solubility ]
Methanol (Slightly), Water (Slightly) | [Water Solubility ]
Soluble in water | [Colour Index ]
42585 | [form ]
Liquid | [color ]
Clear blue-green | [PH Range]
0.1(yellow)-2.3(blue) | [Stability:]
Hygroscopic | [CAS DataBase Reference]
82-94-0 | [EPA Substance Registry System]
C.I. Basic Blue 20 (82-94-0) |
| Hazard Information | Back Directory | [Definition]
ChEBI: An iminium salt composed of (4-{[4-(dimethylamino)phenyl][4-(trimethylazaniumyl)phenyl]methylidene}cyclohexa-2,5-dien-1-ylidene)(dimethyl)ammonium and chloride ions in a 1:2 ratio. A histological dye used to demonstrate nucleic acids by the Unna-Pappenheim
stain, in conjunction with Pyronin Y. | [Description]
Methyl Green is a DNA stain used as a tracking dye in acid buffers for electrophoresis. It helps differentiate bacteria and is also a tissue stain for nucleoli and mitochondria in tissues. It is used with Bismarck Brown frequently. | [Chemical Properties]
Methyl green is a metallic green microcrystal or bright green powder. It is soluble in water and appears bluish-green. As a basic dye, it readily binds to highly polymerized DNA, producing a green color. It is also known as Double Green SF. It appears as green crystals with a golden luster or as a light green powder. It is soluble in water and appears bluish-green. It is slightly soluble in ethanol but insoluble in ether or amyl alcohol.
Methyl green exists in two forms. Formula I: Molecular formula C26H33Cl2N3. Molecular weight 458.49. It appears as green crystals with a golden luster or as a light green powder. It is soluble in water, slightly soluble in ethanol, and insoluble in ether. It is typically a complex salt of zinc chloride. Formula II: Molecular formula: C27H35BrClN3. Molecular weight: 516.98. Green powder, soluble in water, exhibits a greenish-blue color. The solution is yellow at pH 0.2 and blue at pH 1.8. | [Uses]
Methyl Green powder dye is used in various staining methods in microscopy. It is also used with Pyronine Y dye for one stage DNA (green) and RNA (red) staining method. It is also used as a counterstain with other reactions, such as demonstration of enzymatic activity and for mitochondrial staining according to Altmann. Methyl green solution is a suitable counterstain for chloroacetate esterase, nonspecific esterase, alkaline phosphatase, peroxidase, naphthol AS acetate esterase, and acid phosphatase. | [Preparation]
chlorine methane and (C.I.Basic Violet 1, C.I. 42535) reaction | [Properties and Applications]
green light blue. Soluble in water for blue light green, not soluble in ethanol and e alcohol. The strong sulfuric acid to red light yellow, yellow green for diluted. Dyes to join sodium hydroxide solution to colorless.
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Standard( Cotton )
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Light Fastness
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Persperation Fastness
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Ironing Fastness
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Soaping
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Fading
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Stain
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Fading
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Stain
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Fading
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Stain
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C
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1
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2
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| [Purification Methods]
Crystallise the dye from hot water. [Beilstein 13 IV 2286.] |
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| Company Name: |
Energy Chemical
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| Tel: |
021-021-58432009 400-005-6266 |
| Website: |
http://www.energy-chemical.com |
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