| Identification | Back Directory | [Name]
CTC | [CAS]
90217-02-0 | [Synonyms]
CTC
Ctc (biological Stain)
Cyanotolyl Tetrazolium, Ctc
CYANODITOLYL TETRAZOLIUM CHLORIDE
CYANOTOLYL TETRAZOLIUM CHLORIDE (CTC)
5-CYANO-2,3-DITOLYL TETRAZOLIUM CHLORIDE
CTC 5-Cyano-2,3-ditolyl tetrazolium chloride
5-CYANO-2,3-DI-(P-TOLYL)TETRAZOLIUM CHLORIDE
5-Cyano-2,3-bis(4-methylphenyl)tetrazolium chloride
5-CYANO-2,3-BIS(4-METHYLPHENYL)-2H-TETRAZOLIUM CHLORIDE
2h-tetrazolium, 5-cyano-2,3-bis(4methylphenyl)-, Chloride
5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride, ≥85% (HPLC) | [Molecular Formula]
C16H14ClN5 | [MDL Number]
MFCD00467521 | [MOL File]
90217-02-0.mol | [Molecular Weight]
311.77 |
| Chemical Properties | Back Directory | [Melting point ]
225 ℃ | [storage temp. ]
2-8°C | [solubility ]
Soluble in water | [form ]
powder | [color ]
Off-white | [λmax]
450nm (of formazan) | [BRN ]
8954451 | [Biological Applications]
NADPH-cytochrome P 450 reductase activity assays; detecting gamma-hydroxybutyric acid (GHB),metabolic activity,microorganisms; localizing glucose-6-phosphate dehydrogenase activity; monitoring respiratory activity; treating cancer | [InChI]
InChI=1S/C16H15N5.ClH/c1-13-3-7-15(8-4-13)19-12-18-20(11-17)21(19)16-9-5-14(2)6-10-16;/h3-10,12H,1-2H3;1H | [InChIKey]
BLCCXPMHHCWJHC-UHFFFAOYSA-N | [SMILES]
N1(C2=CC=C(C)C=C2)C=NN(C#N)[NH+]1C1=CC=C(C)C=C1.[Cl-] |
| Hazard Information | Back Directory | [Description]
5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms. This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm). While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm; emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter. For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable. | [Uses]
5-Cyano-2,3-di-p-tolyltetrazolium Chloride is a monotetrazolium redox dye that is used as a vital stain for actively respiring bacteria. Redox dye producing a fluorescent formazan (only in solid state) upon reduction; used as cellular indicator of respiratory activity in cells. | [Uses]
Redox dye producing a fluorescent formazan (only in solid state) upon reduction. It is used as cellular indicator of respiratory activity in cells, especiall for tracking P450 activity. | [References]
[1] G G RODRIGUEZ. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria.[J]. Applied and Environmental Microbiology, 1992, 58 6: 1801-1808. DOI: 10.1128/aem.58.6.1801-1808.1992
[2] G. CARUSO E. C M Mancuso. Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters[J]. Journal of Applied Microbiology, 2003, 95 2: 225-233. DOI: 10.1046/j.1365-2672.2003.01967.x
[3] M E SIERACKI J N T L Cucci. Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures.[J]. Applied and Environmental Microbiology, 1999, 65 6: 2409-2417. DOI: 10.1128/aem.65.6.2409-2417.1999
[4] VISHVESH K BHUPATHIRAJU . Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria[J]. Journal of microbiological methods, 1999, 37 3: Pages 231-243. DOI: 10.1016/s0167-7012(99)00069-x |
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