| Identification | Back Directory | [Name]
BIOCYTIN HYDROCHLORIDE | [CAS]
98930-70-2 | [Synonyms]
nε-biotinyl-l-lysine
BIOCYTIN HYDROCHLORIDE
BIOCYTIN HYDROCHLORIDE 98%
Biocytin hydrochloride >=98% (TLC)
N6-[5-((3aS)-2-oxo-(3ar,6ac)-hexahydro-thieno[3,4-d]imidazol-4t-yl)-valeryl]-L-lysine
(S)-2-aMino-6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]iMidazol-4-yl)pentanaMido)hexanoic acid hydrochloride | [Molecular Formula]
C16H29ClN4O4S | [MDL Number]
MFCD00136057 | [MOL File]
98930-70-2.mol | [Molecular Weight]
408.94 |
| Chemical Properties | Back Directory | [storage temp. ]
−20°C | [solubility ]
good in water, DMSO | [form ]
powder | [color ]
white to off-white | [Appearance]
white powder | [InChI]
1S/C16H28N4O4S.ClH/c17-10(15(22)23)5-3-4-8-18-13(21)7-2-1-6-12-14-11(9-25-12)19-16(24)20-14;/h10-12,14H,1-9,17H2,(H,18,21)(H,22,23)(H2,19,20,24);1H | [InChIKey]
FMGQMNFQNPUYBS-UHFFFAOYSA-N | [SMILES]
Cl.NC(CCCCNC(=O)CCCCC1SCC2NC(=O)NC12)C(O)=O |
| Hazard Information | Back Directory | [Description]
Biocytin (ε-biotinoyl-L-lysine) is a derivative of D-biotin and L-lysine. The compound has both a carboxyl group and an amino group, due to which it is widely used as a spacer and a component of trifunctional crosslinkers in the modification of proteins.
Biocytin, as a biotinidase cofactor, can be used to determine the specificity of biotinidases and to study the kinetics and mechanisms of cellular delivery.
Biocytin is also used as an anterograde neurotracer.
Biocytin hydrochloride has a high solubility in aqueous solutions; it can be used to biotinylate proteins under slightly acidic conditions (pH 4-6) using EDAC, which distinguishes it from N-hydroxysuccinimide (NHS) esters of biotin. | [Biological Activity]
Biocytin is used as a substrate to study the specificity and kinetics of biotinidase(s)to measure biotinidase deficiency and as a model compound to study various uptake mechanisms of cells and tissues. |
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