1644388-26-0
1644388-26-0 结构式
基本信息
英文别名
EAD1 HClEAD1(free base)
1,2-Ethanediamine, N1-[2-[[[1-[(4-chlorophenyl)methyl]-1H-1,2,3-triazol-4-yl]methyl]amino]ethyl]-N2-(7-chloro-4-quinolinyl)-N1-methyl-
常见问题列表
生物活性
EAD1 是一种有效的自噬 (autophagy) 抑制剂，在肺癌和胰腺癌细胞中具有抗增殖活性。EAD1 还诱导凋亡 (apoptosis)。体外研究
EAD1 (0-200 μM; 72 hours) has antiproliferative activity against human cancer cell lines.
EAD1 (5-75 μM; 24 hours) induces apoptosis in the H460 cells in a concentration-dependent manner.
EAD1 (1-25 μM; 24 hour) increases the punctate LC3 signal in H460 cells.
Cell Proliferation Assay
| Cell Line: | H460 and HCC827 nonsmall cell lung cancer (NSCLC) and BxPC3 pancreatic cancer cells |
| Concentration: | 0, 50, 100, 150, 200 μM |
| Incubation Time: | 72 hours |
| Result: | IC 50 s of 11, 7.6, and 5.8 μM for H460, HCC827, and BxPC3 cells, respectively. |
Apoptosis Analysis
| Cell Line: | H460 cells |
| Concentration: | 5, 25, 50, and 75 μM |
| Incubation Time: | 24 hours |
| Result: | Induction of apoptosis. |
Western Blot Analysis
| Cell Line: | H460 cells |
| Concentration: | 1, 2.5, 5, 10, 25 μM |
| Incubation Time: | 24 hours |
| Result: | Caused concentration and time-dependent increases in LC3-II levels. |