23313-12-4
23313-12-4 结构式
基本信息
维生素C磷酸酯(L-抗坏血酸-2-磷酸酯)
Ascorbyl-2-phosphate
ascorbate-2-phosphate
Ascorbyl monophosphate
Ascorbic Acid Impurity 6
Ascorbyl-2-monophosphate
2-Phospho-L-ascorbic acid
MIJPAVRNWPDMOR-ZAFYKAAXSA-N
L-Ascorbic acid, 2-(dihydrogen phosphate)
l-ascorbic acid 2-monophosphate tris-cyclohexylammonium salt
物理化学性质
常见问题列表
IC50: alkaline phosphatase (ALP); collagen formation; runx2A
L-Ascorbic acid 2-phosphate (0.1-1.5 mM; 2 to 3 weeks with medium exchange every 2 to 3 days) significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) achieves only weak growth stimulation. A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect . L-Ascorbic acid 2-phosphate (50 µM-250 µM) is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 µM AsA2-P and 10 nM dexamethasone (Dex), and 250 µM AsA2-P and 5 nM Dex.
Cell Proliferation Assay
| Cell Line: | Human corneal endothelial cells (HCECs) |
| Concentration: | 0.1 mM; 0.3 mM; 1.5 mM |
| Incubation Time: | 2 to 3 weeks with medium exchange every 2 to 3 days |
| Result: | Stimulated HCEC cells growth. |