ANTI-NF-KB P65抗体

ANTI-NF-KB P65抗体性质、用途与生产工艺

描述

Anti-NF-kB p65 Antibody, Mouse Monoclonal

宿主

Mouse

应用

ELISA,FCM,ICC/IF

种属反应性

Human

制备方法

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RELA / Transcription factor p65 (rh RELA / Transcription factor p65; Catalog#12054-H09E; Q04206-1; Met 1-Tyr 306). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

品牌示例

Anti-NF-kB p65 Antibody, Mouse Monoclonal---Cat: 12054-MM04

Immunofluorescence staining of human RELA (NF-KB p65) in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-human RELA monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

背景

RELA (v-rel reticuloendotheliosis viral oncogene homolog A), also known as Nuclear factor NF-kappa-B p65 subunit, or Transcription factor p65, is a transcription factor expressed in growth plate chondrocytes where it facilitates chondrogenesis. The v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) gene encodes the major component of the NF-?B complex. NF-kappaB is a generic name for an evolutionarily conserved transcription-factor system that contributes to the mounting of an effective immune response but is also involved in the regulation of cell proliferation, development, and apoptosis. The implication of NF-kappaB in central biological processes and its extraordinary connectivity to other signaling pathways raise a need for highly controlled regulation of NF-kappaB activity at several levels. The mammalian Rel/NF-kappaB family of transcription factors, including RelA, c-Rel, RelB, NF-kappaB1 (p5 and its precursor p15), and NF-kappaB2 (p52 and its precursor p1), plays a central role in the immune system by regulating several processes ranging from the development and survival of lymphocytes and lymphoid organs to the control of immune responses and malignant transformation.

靶点背景

NF-κB（核因子激活的B细胞的κ-轻链增强）是一种蛋白质复合物，其控制转录的DNA，细胞因子产生和细胞存活。NF-κB几乎存在于所有动物细胞类型中，并参与细胞对刺激的反应，如应激，细胞因子，自由基，重金属，紫外线照射，氧化LDL和细菌或病毒抗原。NF-κB在调节对感染的免疫应答中起关键作用。NF-κB的不正确调节与癌症，炎症和自身免疫疾病，感染性休克，病毒感染和免疫发育不当有关。NF-κB也与突触可塑性和记忆过程有关。

下游应用

ANTI-NF-KB P65抗体是一类可以特异性结合ANTI-NF-KB P65的克隆抗体，主要用于检测ANTI-NF-KB P65的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。

ANTI-NF-KB P65抗体 上下游产品信息

上游原料

下游产品

ANTI-NF-KB P65抗体 生产厂家

 

ANTI-NF-KB P65抗体 相关搜索:

• ANTI-NF-KB P65抗体
• Anti-NF-kB p65 Antibody