Anti-Luciferase antibody, Mouse monoclonal Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Anti-Luciferase antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunocytochemistry
- immunohistochemistry
Allgemeine Beschreibung
Anti-Luciferase antibody, Mouse monoclonal , (mouse IgG1 isotype) is derived from the LUC-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with firefly (Photinus pyralis) luciferase. Luciferase, a reporter protein contains two binding pockets, one pocket for ATP and other one for luciferin substrate. Both the pockets are in close proximity to each other.
Biochem/physiol Actions
Luciferase catalyzes a bioluminescent reaction in the presence of substrate luciferin as well as Mg2+, oxygen and ATP. The luciferase assay is rapid, sensitive, and unlike the CAT assay, does not require a radioactive substrate. Anti-Luciferase antibody can detect the luciferase enzyme expression in situ, providing a means to study the cellular localization of the signal sequence activation.
Anti-Luciferase antibody, Mouse monoclonal Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
