ATTOPHOS Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
AttoPhos has been used to detect bound probes by an image capture system.
It has also been used as a fluorescence substrate in the study to detect antigens by quantitative immuno-PCR (polymerase chain reaction).
Allgemeine Beschreibung
AttoPhos is a highly sensitive fluorimetric substrate for the detection of alkaline phosphatase. With an optimal excitation wavelength of 430–440 nm and the emission maximum at 560 nm, an exceptionally large Stokes Shift is seen. This results in an extremely low intrinsic background signal with a detection limit of 10–19 moles for alkaline phosphatase. In addition, the background fluorescence caused by biological molecules (assay components), cuvettes, or microplates is greatly reduced. With these unique properties, AttoPhos is more sensitive by a factor of 100 than the most commonly used fluorescence substrate 4-methylumbelliferyl-phosphate (MUP).
ATTOPHOS Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
