Sulfo-SMPB (sulfosuccinimidyl 4-(N-maleimidophenyl)butyrate) Chemische Eigenschaften,Einsatz,Produktion Methoden
Allgemeine Beschreibung
SMPB and its water-soluble analog Sulfo-SMPB are heterobifunctional crosslinkers that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7-9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. In aqueous solutions, hydrolytic degradation of the NHS ester is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group but will slowly hydrolyze and also lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugation experiments involving these crosslinkers are usually performed at pH 7.2-7.5, with the NHS-ester (amine-targeted) reaction being accomplished before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.
Sulfo-SMPB (sulfosuccinimidyl 4-(N-maleimidophenyl)butyrate) Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
