Primer for cDNA Synthesis Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
Primer for cDNA synthesis may be used in:
- RT-PCR
- Generation of cDNA libraries
- Synthesis of first-strand cDNA
Allgemeine Beschreibung
This primer consists of a 15-mer oligonucleotide that shows thymidine (T) at each individual position. The oligonucleotides are HPLC purified, desalted, and supplied as a lyophilizate of the Li-salt. The p(dT)
15 primer is not phosphorylated at the 5′ end.Oligo(dT)N generates full-length cDNAs. If template contains oligo(A) stretches internally, the primer may bind these, causing mis priming and transcripts that are not full-length.
Composition: p(dT)
15[ 15-mer oligonucleotide containing only deoxythymidine (dT) residues].
Phosphorylation: The p(dT)
15 primer is not phosphorylated at the 5′ end.
Primer for cDNA Synthesis Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
