KAPA2G Robust HotStart PCR Kit

KAPA2G Robust HotStart PCR Kit Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

KAPA2G Robust HotStart^? PCR Kit has been used in:

• PCR amplification of saliva samples
• PCR amplification for direct sequencing
• Nested PCR
• Amplification of GC- or AT-rich templates
• Templates containing common PCR inhibitors e.g. salts, urea, SDS, or ethanol
• Crude sample PCR e.g. buccal swabs, cultured mammalian, yeast, or bacterial cells
• Colony PCR
• RT-PCR

Allgemeine Beschreibung

The second-generation KAPA2G Robust DNA Polymerase was evolved to solve inconsistent amplification across a broad range of amplicon types (GC- and AT-rich). KAPA2G Robust HotStart PCR Kit enables higher processivity and specific activity, which translates to robust PCR performance, high sensitivity, and improved tolerance to common inhibitors. The high performance of the KAPA2G Robust DNA Polymerase is ideally suited for challenging PCR applications and difficult samples, eliminating the need for optimization using multiple enzymes and protocols.

Biochem/physiol Actions

KAPA2G Robust HotStart? DNA Polymerase generated DNA are similar to fragments generated with wild-type Taq DNA polymerase. The fidelity of KAPA2G Robust HotStart is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated. It may be used in restriction enzyme digestion, cloning, and sequencing. Like wild-type Taq, KAPA2G Robust HotStart has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity.

KAPA2G Robust HotStart PCR Kit Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

KAPA2G Robust HotStart PCR Kit Anbieter Lieferant Produzent Hersteller Vertrieb Händler.

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51471	80

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• KAPA2G Robust HotStart PCR Kit