KAPA Taq PCR Kit Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
KAPA Taq PCR Kit has been used in:
- High throughput PCR
- Amplification of low copy DNA templates
- Multiplex PCR
- Specific amplification of complex templates
- RT-PCR
- random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)
- Polymerase chain reaction (PCR)
- Genotyping
Biochem/physiol Actions
KAPA Taq PCR Kit, which contains KAPA Taq DNA Polymerase, is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. KAPA Taq and KAPA Taq HotStart? DNA Polymerase have 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′ → 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated. In the hot start formulation, the KAPA Taq is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.
KAPA Taq PCR Kit Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
